Analysis Of Regulatory Elements Of The Promoter And The 3' Untranslated Region Of The Maize Hrgp Gene Coding For A Cell Wall Protein.

dc.creatorMenossi, M
dc.creatorRabaneda, F
dc.creatorPuigdomènech, P
dc.creatorMartínez-Izquierdo, J A
dc.date2003-Jun
dc.date2015-11-27T12:52:11Z
dc.date2015-11-27T12:52:11Z
dc.date.accessioned2018-03-29T00:57:31Z
dc.date.available2018-03-29T00:57:31Z
dc.identifierPlant Cell Reports. v. 21, n. 9, p. 916-23, 2003-Jun.
dc.identifier0721-7714
dc.identifier10.1007/s00299-003-0602-0
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12789511
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195407
dc.identifier12789511
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295640
dc.descriptionHydroxyproline-rich glycoproteins (HRGP) are structural components of the plant cell wall. Hrgp genes from maize and related species have a conserved 500 bp sequence in the 5'-flanking region, and all Hrgp genes from monocots have an intron located in the 3' untranslated region. To study the role of these conserved regions, several deletions of the Hrgp gene were fused to the beta-glucuronidase ( GUS) gene and used to transform maize tissues by particle bombardment. The overall pattern of GUS activity directed by sequential deletions of the Hrgp promoter was different in embryos and young shoots. In embryos, the activity of the full-length Hrgp promoter was in the same range as that of the p35SI promoter construct, based on the strong 35S promoter, whereas in the fast-growing young shoots it was 20 times higher. A putative silencer element specific for young shoots was found in the -1,076/-700 promoter region. Other major cis elements for Hrgp expression are probably located in the regions spanning -699/-510 and -297/-160. Sequences close to the initial ATG and mRNA leader were also important since deletion of the region -52/+16 caused a 75% reduction in promoter activity. The presence of the Hrgp intron in the 3' untranslated region changed the levels of GUS activity directed by the Hrgp and the 35S promoters. This pattern of activity was complex, and was dependent on the promoter and cell type analysed.
dc.description21
dc.description916-23
dc.languageeng
dc.relationPlant Cell Reports
dc.relationPlant Cell Rep.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subject3' Untranslated Regions
dc.subjectBase Sequence
dc.subjectBiolistics
dc.subjectDna, Plant
dc.subjectGlycoproteins
dc.subjectMolecular Sequence Data
dc.subjectPlant Proteins
dc.subjectPromoter Regions, Genetic
dc.subjectRegulatory Sequences, Nucleic Acid
dc.subjectSequence Deletion
dc.subjectZea Mays
dc.titleAnalysis Of Regulatory Elements Of The Promoter And The 3' Untranslated Region Of The Maize Hrgp Gene Coding For A Cell Wall Protein.
dc.typeArtículos de revistas


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