Comparative Cytotoxicity Of Dimethylamide-crotonin In The Promyelocytic Leukemia Cell Line (hl60) And Human Peripheral Blood Mononuclear Cells.

dc.creatorAnazetti, Maristella Conte
dc.creatorMelo, Patricia Silva
dc.creatorDurán, Nelson
dc.creatorHaun, Marcela
dc.date2003-Jun
dc.date2015-11-27T12:52:09Z
dc.date2015-11-27T12:52:09Z
dc.date.accessioned2018-03-29T00:57:29Z
dc.date.available2018-03-29T00:57:29Z
dc.identifierToxicology. v. 188, n. 2-3, p. 261-74, 2003-Jun.
dc.identifier0300-483X
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12767696
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195396
dc.identifier12767696
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295629
dc.descriptionDehydrocrotonin (DHC) is a diterpene lactone obtained from Croton cajucara (Sacaca). Dimethylamide-crotonin (DCR), a DHC derivative, has a similar inhibitory effect on leukemic HL60 cells than its parent compound evaluated by different endpoints of cytotoxicity. No cytotoxicity or morphological alterations associated with apoptosis were detected in human peripheral blood mononuclear cells (PBMC) after treatment with up to 400 micro M DCR in presence of phytohemaglutinin (5 micro g/ml). Based on morphological changes and the pattern of DNA fragmentation, DHC and DCR were found to induce apoptosis and terminal differentiation (assessed by nitro blue tetrazolium reduction) in HL60 cells, but these compounds did not show any toxic effect in PBMC. Thus, DCR and DHC inhibit HL60 cell growth in vitro partly by inducing apoptosis and cell differentiation, but does not cause serious damage to immune cells according to our experimental conditions.
dc.description188
dc.description261-74
dc.languageeng
dc.relationToxicology
dc.relationToxicology
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAntineoplastic Agents, Phytogenic
dc.subjectApoptosis
dc.subjectCell Differentiation
dc.subjectCell Division
dc.subjectDna Fragmentation
dc.subjectDiterpenes
dc.subjectDiterpenes, Clerodane
dc.subjectElectrophoresis, Agar Gel
dc.subjectHl-60 Cells
dc.subjectHumans
dc.subjectLactones
dc.subjectLeukocytes, Mononuclear
dc.subjectNitroblue Tetrazolium
dc.subjectPlants, Medicinal
dc.titleComparative Cytotoxicity Of Dimethylamide-crotonin In The Promyelocytic Leukemia Cell Line (hl60) And Human Peripheral Blood Mononuclear Cells.
dc.typeArtículos de revistas


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