Genotyping Of Human Cytomegalovirus Using Non-radioactive Single-strand Conformation Polymorphism (sscp) Analysis.

dc.creatorde Albuquerque, Dulcinéia Martins
dc.creatorCosta, Sandra Cecília Botelho
dc.date2003-Jun
dc.date2015-11-27T12:52:08Z
dc.date2015-11-27T12:52:08Z
dc.date.accessioned2018-03-29T00:57:27Z
dc.date.available2018-03-29T00:57:27Z
dc.identifierJournal Of Virological Methods. v. 110, n. 1, p. 25-8, 2003-Jun.
dc.identifier0166-0934
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12757917
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195388
dc.identifier12757917
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295621
dc.descriptionGenetic variation in the glycoprotein B (gB) gene may play a role in human cytomegaloviruses (HCMVs) pathogenesis. Using restriction analysis of the gB gene product (PCR-RFLP), amplified by the nested polymerase chain reaction, the HCMV strains can be compared and classified into at least four HCMV groups. PCR single-strand conformation polymorphism (PCR-SSCP) is one of the techniques used to identify a mutant sequence or a polymorphism in a known gene. SSCP analysis has the advantage over RFLP analysis on detection of DNA polymorphisms and point mutations at a variety of positions in DNA fragments. However, the original SSCP protocols using the incorporation of radioactive label and polyacrylamide gel electrophoresis for detection are labour intensive and time-consuming. A simplified SSCP protocol is described to identify HCMV strains and the gB genotype, allowing the detection of sequence variations not residing in the endonuclease recognition sites.
dc.description110
dc.description25-8
dc.languageeng
dc.relationJournal Of Virological Methods
dc.relationJ. Virol. Methods
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectBone Marrow Transplantation
dc.subjectCytomegalovirus
dc.subjectCytomegalovirus Infections
dc.subjectGenetic Variation
dc.subjectGenotype
dc.subjectHumans
dc.subjectPoint Mutation
dc.subjectPolymerase Chain Reaction
dc.subjectPolymorphism, Restriction Fragment Length
dc.subjectPolymorphism, Single-stranded Conformational
dc.subjectReproducibility Of Results
dc.subjectSensitivity And Specificity
dc.subjectViral Envelope Proteins
dc.titleGenotyping Of Human Cytomegalovirus Using Non-radioactive Single-strand Conformation Polymorphism (sscp) Analysis.
dc.typeArtículos de revistas


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