The Hansenula Polymorpha Mox Gene Presents Two Alternative Transcription Start Points Differentially Utilized And Sensitive To Respiratory Activity.

dc.creatorGenu, Victor
dc.creatorGödecke, Stefanie
dc.creatorHollenberg, Cornelis P
dc.creatorPereira, Gonçalo G
dc.date2003-Jun
dc.date2015-11-27T12:52:08Z
dc.date2015-11-27T12:52:08Z
dc.date.accessioned2018-03-29T00:57:27Z
dc.date.available2018-03-29T00:57:27Z
dc.identifierEuropean Journal Of Biochemistry / Febs. v. 270, n. 11, p. 2467-75, 2003-Jun.
dc.identifier0014-2956
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12755702
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195387
dc.identifier12755702
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295620
dc.descriptionThe peroxisomal methanol metabolism of Hansenula polymorpha depends on a group of genes that are coordinately regulated. Methanol oxidase (Mox) plays a key role in this pathway and its synthesis has been shown to be regulated at the transcriptional level. MOX expression is strongly repressed on glucose and activated on glycerol or methanol. In this study we have identified two MOX transcripts that are differentially expressed along MOX derepression. The first one, named l-MOX (for longer MOX), starts at position -425, is only weakly and transiently transcribed and is not translated into the Mox protein. The other is the true MOX mRNA, which initiates around position -25. Using a strain bearing multiple copies of MOX(Q1N) and a reporter gene fused to the MOX promoter, regulation of the two transcripts was investigated. Initiation of the true MOX correlates with repression of l-MOX and conditions that are repressive for MOX transcription, such as the inhibition of mitochondrial activity, lead to higher levels of l-MOX expression. This effect was first observed in a mox mutant (Q1N-M8) unable to grow on nonfermentable carbon sources. No function was detected for l-MOX, but its regulation follows a pattern similar to that of catalase, which is essential for methanol metabolism. This suggests that, l-MOX, although precisely regulated, seems to be a remnant of the evolution of the methanol metabolism network.
dc.description270
dc.description2467-75
dc.languageeng
dc.relationEuropean Journal Of Biochemistry / Febs
dc.relationEur. J. Biochem.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAlcohol Oxidoreductases
dc.subjectAntifungal Agents
dc.subjectAntimycin A
dc.subjectBlotting, Northern
dc.subjectGene Deletion
dc.subjectGene Expression Regulation, Enzymologic
dc.subjectGene Expression Regulation, Fungal
dc.subjectGenes, Fungal
dc.subjectGenes, Reporter
dc.subjectGlucose
dc.subjectGlycerol
dc.subjectKinetics
dc.subjectMethanol
dc.subjectMitochondria
dc.subjectMutation
dc.subjectOxygen Consumption
dc.subjectPeroxisomes
dc.subjectPichia
dc.subjectPlasmids
dc.subjectPromoter Regions, Genetic
dc.subjectRna, Messenger
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectTranscription, Genetic
dc.titleThe Hansenula Polymorpha Mox Gene Presents Two Alternative Transcription Start Points Differentially Utilized And Sensitive To Respiratory Activity.
dc.typeArtículos de revistas


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