Novel Signal Transduction Pathway For Luteinizing Hormone And Its Interaction With Insulin: Activation Of Janus Kinase/signal Transducer And Activator Of Transcription And Phosphoinositol 3-kinase/akt Pathways.

dc.creatorCarvalho, Carla R O
dc.creatorCarvalheira, José B C
dc.creatorLima, Maria H M
dc.creatorZimmerman, Suzana F
dc.creatorCaperuto, Luciana C
dc.creatorAmanso, Angélica
dc.creatorGasparetti, Alessandra L
dc.creatorMeneghetti, Vainer
dc.creatorZimmerman, Leonardo F
dc.creatorVelloso, Licio A
dc.creatorSaad, Mario J A
dc.date2003-Feb
dc.date2015-11-27T12:52:04Z
dc.date2015-11-27T12:52:04Z
dc.date.accessioned2018-03-29T00:57:20Z
dc.date.available2018-03-29T00:57:20Z
dc.identifierEndocrinology. v. 144, n. 2, p. 638-47, 2003-Feb.
dc.identifier0013-7227
dc.identifier10.1210/en.2002-220706
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12538627
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195358
dc.identifier12538627
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295591
dc.descriptionThe actions of LH are mediated through a single class of cell surface LH/human chorionic gonadotropin receptor, which is a member of the G protein-coupled receptor family. In the present study we showed that LH induced rapid tyrosine phosphorylation and activation of the Janus kinase 2 (JAK2) in rat ovary. Upon JAK2 activation, tyrosine phosphorylation of signal transducer and activator of transcription-1 (STAT-1), STAT-5b, insulin receptor substrate-1 (IRS-1), and Src homology and collagen homology (Shc) were detected. In addition, LH induced IRS-1/phosphoinositol 3-kinase and Shc /growth factor receptor-binding protein 2 (Grb2) associations and downstream AKT (protein kinase B, homologous to v-AKT) serine phosphorylation and ERK tyrosine phosphorylation, respectively. The simultaneous infusion of insulin and LH induced higher phosphorylation levels of JAK2, STAT5b, IRS-1, and AKT compared with each hormone alone in the whole ovary of normal rats. By immunohistochemistry we demonstrated that these late events take place in follicular cells and both external and internal theca. These results indicate a new signal transduction pathway for LH and show that there is positive cross-talk between the insulin and LH signaling pathways at the level of phosphoinositol 3-kinase/AKT pathway in this tissue.
dc.description144
dc.description638-47
dc.languageeng
dc.relationEndocrinology
dc.relationEndocrinology
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectDna-binding Proteins
dc.subjectFemale
dc.subjectInsulin
dc.subjectInsulin Receptor Substrate Proteins
dc.subjectJanus Kinase 2
dc.subjectLuteinizing Hormone
dc.subjectMilk Proteins
dc.subjectMitogen-activated Protein Kinases
dc.subjectOvary
dc.subjectPhosphatidylinositol 3-kinases
dc.subjectPhosphoproteins
dc.subjectPhosphorylation
dc.subjectProtein-serine-threonine Kinases
dc.subjectProtein-tyrosine Kinases
dc.subjectProto-oncogene Proteins
dc.subjectProto-oncogene Proteins C-akt
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReceptor Cross-talk
dc.subjectStat1 Transcription Factor
dc.subjectStat5 Transcription Factor
dc.subjectSignal Transduction
dc.subjectTrans-activators
dc.subjectTyrosine
dc.subjectSrc Homology Domains
dc.titleNovel Signal Transduction Pathway For Luteinizing Hormone And Its Interaction With Insulin: Activation Of Janus Kinase/signal Transducer And Activator Of Transcription And Phosphoinositol 3-kinase/akt Pathways.
dc.typeArtículos de revistas


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