Induction Of Protein Catabolism And The Ubiquitin-proteasome Pathway By Mild Oxidative Stress.

dc.creatorGomes-Marcondes, Maria Cristina C
dc.creatorTisdale, Michael J
dc.date2002-Jun
dc.date2015-11-27T12:49:34Z
dc.date2015-11-27T12:49:34Z
dc.date.accessioned2018-03-29T00:57:05Z
dc.date.available2018-03-29T00:57:05Z
dc.identifierCancer Letters. v. 180, n. 1, p. 69-74, 2002-Jun.
dc.identifier0304-3835
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/11911972
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195297
dc.identifier11911972
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295530
dc.descriptionMuscle wasting in cancer cachexia is associated with increased levels of malondialdehyde (MDA) in gastrocnemius muscles, suggesting an increased oxidative stress. To determine whether oxidative stress contributes to muscle protein catabolism, an in vitro model system, consisting of C2C12 myotubes, was treated with either 0.2 mM FeSO4, 0.1 mM H2O2, or both, to replicate the rise in MDA content in cachexia. All treatments caused an increased protein catabolism and a decreased myosin expression. There was an increase in the proteasome chymotrypsin-like enzyme activity, while immunoblotting showed an increased expression of the 20S proteasome alpha-subunits, p42, and the ubiquitin-conjugating enzyme, E214k. These results show that mild oxidative stress increases protein degradation in skeletal muscle by causing an increased expression of the major components of the ubiquitin-proteasome pathway.
dc.description180
dc.description69-74
dc.languageeng
dc.relationCancer Letters
dc.relationCancer Lett.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBlotting, Western
dc.subjectCachexia
dc.subjectCells, Cultured
dc.subjectCysteine Endopeptidases
dc.subjectEgg Proteins
dc.subjectMalondialdehyde
dc.subjectMembrane Glycoproteins
dc.subjectMice
dc.subjectMultienzyme Complexes
dc.subjectMuscle, Skeletal
dc.subjectMyosins
dc.subjectOxidative Stress
dc.subjectProteasome Endopeptidase Complex
dc.subjectProteins
dc.subjectReceptors, Cell Surface
dc.subjectTime Factors
dc.subjectUbiquitin
dc.titleInduction Of Protein Catabolism And The Ubiquitin-proteasome Pathway By Mild Oxidative Stress.
dc.typeArtículos de revistas


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