Effect Of Iodide On Fas, Fas-ligand And Bcl-w Mrna Expression In Thyroid Of Nod Mice Pretreated With Methimazole.

dc.creatorBoechat, L H B
dc.creatorVilella, C A
dc.creatorZollner, R L
dc.date2002-Mar
dc.date2015-11-27T12:49:33Z
dc.date2015-11-27T12:49:33Z
dc.date.accessioned2018-03-29T00:57:04Z
dc.date.available2018-03-29T00:57:04Z
dc.identifierBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 35, n. 3, p. 289-95, 2002-Mar.
dc.identifier0100-879X
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/11887206
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195290
dc.identifier11887206
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295523
dc.descriptionNonobese diabetic (NOD) mice and a derived strain, NOD.H.2h4, have been used as a model for experimental spontaneous thyroiditis and thyroiditis induced by iodide excess after a goiter-inducing period. Some authors have proposed that iodide, given after methimazole or propylthiouracil, is capable of inducing apoptosis in thyroid cells and that anti-thyroid drugs can modulate the expression of apoptosis components such as Fas and its ligand (Fas-L). Here we evaluated the effect of potassium iodide (20 microg/animal for 4 days, i.p.) given to NOD mice at the 10th week of life after exposure to methimazole (1 mg/ml) in drinking water from the 4th to the 10th week of life. Fas, Fas-L and Bcl-w expression were analyzed semiquantitatively by RT-PCR immediately after potassium iodide administration (group MI44D) or at week 32 (MI32S). Control groups were added at 10 (C10) and 32 weeks (C32), as well as a group that received only methimazole (CM10). An increase in the expression of Fas-L and Bcl-w (P<0.01, ANOVA) was observed in animals of group MI44D, while Fas was expressed at higher levels (P = 0.02) in group C32 (72.89 +/- 47.09 arbitrary units) when compared to group C10 (10.8 +/- 8.55 arbitrary units). Thus, the analysis of Fas-L and Bcl-w expression in the MI44D group and Fas in group C32 allowed us to detect two different patterns of expression of these apoptosis components in thyroid tissue of NOD mice.
dc.description35
dc.description289-95
dc.languageeng
dc.relationBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]
dc.relationBraz. J. Med. Biol. Res.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAntigens, Cd95
dc.subjectAntithyroid Agents
dc.subjectApoptosis
dc.subjectDisease Models, Animal
dc.subjectElectrophoresis
dc.subjectFas Ligand Protein
dc.subjectGene Expression
dc.subjectMale
dc.subjectMembrane Glycoproteins
dc.subjectMethimazole
dc.subjectMice
dc.subjectMice, Inbred Nod
dc.subjectPotassium Iodide
dc.subjectRna, Messenger
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectThyroid Gland
dc.subjectThyroiditis
dc.titleEffect Of Iodide On Fas, Fas-ligand And Bcl-w Mrna Expression In Thyroid Of Nod Mice Pretreated With Methimazole.
dc.typeArtículos de revistas


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