Iron-regulated Proteins In Phanerochaete Chrysosporium And Lentinula Edodes: Differential Analysis By Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis And Two-dimensional Polyacrylamide Gel Electrophoresis Profiles.

dc.creatorHernández-Macedo, Maria L
dc.creatorFerraz, André
dc.creatorRodríguez, Jaime
dc.creatorOttoboni, Laura M M
dc.creatorDe Mello, Maricilda Palandi
dc.date2002-Feb
dc.date2015-11-27T12:49:33Z
dc.date2015-11-27T12:49:33Z
dc.date.accessioned2018-03-29T00:57:03Z
dc.date.available2018-03-29T00:57:03Z
dc.identifierElectrophoresis. v. 23, n. 4, p. 655-61, 2002-Feb.
dc.identifier0173-0835
dc.identifier10.1002/1522-2683(200202)23:4<655::AID-ELPS655>3.0.CO;2-S
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/11870778
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195288
dc.identifier11870778
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295521
dc.descriptionSodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis (2-DE) were used to identify iron-responsive proteins in the white-rot species (Phanerochaete chrysosporium and Lentinula edodes), by comparing the differential patterns of cellular and membrane proteins obtained from iron-sufficient and iron-deficient mycelia. Six cellular proteins induced by iron restriction have been observed in SDS-PAGE for P. chrysosporium and twelve for L. edodes. In 2-DE, the numbers of iron-restricted induced proteins were 12 and 9, respectively, in a resolution range of 15-60 kDa and pI 4.5-8.1. SDS-PAGE for the plasma membrane protein did not show differences, whereas the outer-membrane protein profile showed 6 and 5 proteins induced by iron depletion in P. chrysosporium and L. edodes, respectively. The results presented here are important data to unravel mechanisms of biosynthesis and/or transport of the iron-complexing agents in ligninolytic fungi and to further correlate them to the ligninolytic processes.
dc.description23
dc.description655-61
dc.languageeng
dc.relationElectrophoresis
dc.relationElectrophoresis
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectChrysosporium
dc.subjectElectrophoresis, Gel, Two-dimensional
dc.subjectElectrophoresis, Polyacrylamide Gel
dc.subjectFungal Proteins
dc.subjectGene Expression Regulation, Fungal
dc.subjectIron
dc.subjectShiitake Mushrooms
dc.titleIron-regulated Proteins In Phanerochaete Chrysosporium And Lentinula Edodes: Differential Analysis By Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis And Two-dimensional Polyacrylamide Gel Electrophoresis Profiles.
dc.typeArtículos de revistas


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