Protective Effect Of D-glucose, L-leucine And Fetal Calf Serum Against Oxidative Stress In Neonatal Pancreatic Islets.

dc.creatorStoppiglia, Luiz F
dc.creatorNogueira, Tatiane A
dc.creatorLeite, Adriana R
dc.creatorCarneiro, Everardo M
dc.creatorBoschero, Antonio C
dc.date2002-Nov
dc.date2015-11-27T12:49:20Z
dc.date2015-11-27T12:49:20Z
dc.date.accessioned2018-03-29T00:56:36Z
dc.date.available2018-03-29T00:56:36Z
dc.identifierBiochimica Et Biophysica Acta. v. 1588, n. 2, p. 113-8, 2002-Nov.
dc.identifier0006-3002
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12385774
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195169
dc.identifier12385774
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295402
dc.descriptionB-cell destruction during the onset of diabetes mellitus is associated with oxidative stress. In this work, we investigated the mechanisms of defense against oxidative stress present in neonatal islets and their modulation by D-glucose, L-leucine and fetal calf serum (FCS). Culturing neonatal rat islets in the presence of low D-glucose concentrations (2.8-5.6 mmol/l) and 1 mmol/l H(2)O(2) increased the D-glucose uptake by islets sixfold compared to control levels. This effect was dose-dependently inhibited by D-glucose or FCS and by high concentrations of L-leucine. These supplements allowed islets to increase cytoplasmic catalase (CAT) activity only in response to H(2)O(2), with no decrease in NO formation. Although L-leucine increased CAT activity and restored D-glucose uptake, it did not prevent damage to the islets. These data indicate that the most important H(2)O(2) scavenger system in the islets is CAT and that this system can be modulated by metabolic substrates.
dc.description1588
dc.description113-8
dc.languageeng
dc.relationBiochimica Et Biophysica Acta
dc.relationBiochim. Biophys. Acta
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAnimals, Newborn
dc.subjectCatalase
dc.subjectGlucose
dc.subjectHydrogen Peroxide
dc.subjectIn Vitro Techniques
dc.subjectIslets Of Langerhans
dc.subjectLeucine
dc.subjectNitrites
dc.subjectOxidative Stress
dc.subjectPeroxidase
dc.subjectRats
dc.titleProtective Effect Of D-glucose, L-leucine And Fetal Calf Serum Against Oxidative Stress In Neonatal Pancreatic Islets.
dc.typeArtículos de revistas


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