Clonal Relationships Among Avian Escherichia Coli Isolates Determined By Enterobacterial Repetitive Intergenic Consensus (eric)-pcr.

dc.creatorda Silveira, Wanderley Dias
dc.creatorFerreira, Alessandra
dc.creatorLancellotti, Marcelo
dc.creatorBarbosa, Isildinha A G C D
dc.creatorLeite, Domingos S
dc.creatorde Castro, Antonio F P
dc.creatorBrocchi, Marcelo
dc.date2002-Nov
dc.date2015-11-27T12:49:20Z
dc.date2015-11-27T12:49:20Z
dc.date.accessioned2018-03-29T00:56:36Z
dc.date.available2018-03-29T00:56:36Z
dc.identifierVeterinary Microbiology. v. 89, n. 4, p. 323-8, 2002-Nov.
dc.identifier0378-1135
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12383641
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195167
dc.identifier12383641
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295400
dc.descriptionForty-nine avian Escherichia coli isolates isolated from different outbreak cases of septicemia (24 isolates), swollen head syndrome (14 isolates) and omphalitis (11 isolates), and 30 commensal isolates isolated from poultry with no signs of illness were characterized by enterobacterial repetitive intergenic consensus (ERIC)-PCR technique and their serotypes were determined. The ERIC-PCR profile allowed the typing of the 79 isolates into 68 ERIC-types and grouped the isolates into four main clusters (A-D), with the omphalitis isolates being grouped with the commensals and separated from the septicaemia and swollen head syndrome. These results indicate that ERIC-PCR is a technique that could replace other molecular characterization techniques such as random amplification of polymorphic DNA (RAPD)-PCR and restriction fragment length polymorphism (RFLP), reinforce previous observations that omphalitis isolates are just opportunistic agents, and are consistent with many reports that specific genotypes are responsible for causing specific diseases. Most of the isolates were either nontypable or rough, supporting the need for alternative methods for typing these isolates.
dc.description89
dc.description323-8
dc.languageeng
dc.relationVeterinary Microbiology
dc.relationVet. Microbiol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBrazil
dc.subjectChickens
dc.subjectCluster Analysis
dc.subjectDna Fingerprinting
dc.subjectDna, Bacterial
dc.subjectDna, Intergenic
dc.subjectEscherichia Coli
dc.subjectEscherichia Coli Infections
dc.subjectPolymerase Chain Reaction
dc.subjectPoultry Diseases
dc.titleClonal Relationships Among Avian Escherichia Coli Isolates Determined By Enterobacterial Repetitive Intergenic Consensus (eric)-pcr.
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución