Dot-enzyme Linked Immunosorbent Assay As An Alternative Technique For The Detection Of Bovine Respiratory Syncytial Virus (brsv) Antibodies.

dc.creatorDomingues, Helena Gallicchio
dc.creatorCampalans, Jacqueline
dc.creatorAlmeida, Renata Servan
dc.creatorCoswig, Lia Treptow
dc.creatorArns, Clarice Weis
dc.date
dc.date2015-11-27T12:49:14Z
dc.date2015-11-27T12:49:14Z
dc.date.accessioned2018-03-29T00:56:27Z
dc.date.available2018-03-29T00:56:27Z
dc.identifierVeterinary Research. v. 33, n. 4, p. 397-404
dc.identifier0928-4249
dc.identifier10.1051/vetres:2002025
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12199366
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195126
dc.identifier12199366
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295359
dc.descriptionA dot-ELISA test for the detection of anti-BRSV antibodies is described. The objective of this study was the standardisation of a test as a fast, inexpensive and effective alternative to detect anti-BRSV antibodies. Its sensitivity, specificity and usefulness were compared to a commercial ELISA-kit and to the standard serum neutralisation (SN) test. The standardisation of the technique was done using nitrocellulose disks soaked with a viral sample isolated in Brazil, BRSV-25-BR. The best results were obtained when the disks were sensitised with a purified antigen at a concentration of 0.7 microg/disk and the bovine serum was diluted 1: 200. The experiment used 423 samples of bovine serum collected in the main cattle breeding centres in Brazil. The standard SN, dot-ELISA technique and commercial ELISA kits scored 67.8%, 71.8% and 72.3% of the samples as positive, respectively. When compared to the SN test, the standardised dot-ELISA and the commercial ELISA tests presented relative sensitivities of 92.3% and 91.6% and relative specificities of 71.3% and 68.4% respectively. The results demonstrated that the dot-ELISA test is adequate for the objectives proposed by this study, being easy to use and economically viable. Thus, this test represents an alternative for BRSV serological diagnosis in the substitution of SN and commercial ELISA tests, recommendable for utilisation in laboratories with few resources.
dc.description33
dc.description397-404
dc.languageeng
dc.relationVeterinary Research
dc.relationVet. Res.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAntibodies, Viral
dc.subjectAntigens, Viral
dc.subjectCattle
dc.subjectCattle Diseases
dc.subjectCells, Cultured
dc.subjectEnzyme-linked Immunosorbent Assay
dc.subjectNeutralization Tests
dc.subjectPredictive Value Of Tests
dc.subjectReagent Kits, Diagnostic
dc.subjectRespiratory Syncytial Virus Infections
dc.subjectRespiratory Syncytial Virus, Bovine
dc.subjectSensitivity And Specificity
dc.titleDot-enzyme Linked Immunosorbent Assay As An Alternative Technique For The Detection Of Bovine Respiratory Syncytial Virus (brsv) Antibodies.
dc.typeArtículos de revistas


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