Characterization Of The Hypotensive Effect Of S-nitroso-n-acetylcysteine In Normotensive And Hypertensive Conscious Rats.

dc.creatorRicardo, Kelly Fabiane Santos
dc.creatorShishido, Sílvia Mika
dc.creatorde Oliveira, Marcelo Ganzarolli
dc.creatorKrieger, Marta Helena
dc.date2002-Aug
dc.date2015-11-27T12:49:12Z
dc.date2015-11-27T12:49:12Z
dc.date.accessioned2018-03-29T00:56:23Z
dc.date.available2018-03-29T00:56:23Z
dc.identifierNitric Oxide : Biology And Chemistry / Official Journal Of The Nitric Oxide Society. v. 7, n. 1, p. 57-66, 2002-Aug.
dc.identifier1089-8603
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12175821
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195112
dc.identifier12175821
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295345
dc.descriptionS-Nitrosothiols (RSNOs) are potent vasodilators found naturally in vivo. A variety of synthetic RSNOs have been considered as potential nitric oxide (NO) donors for biomedical applications. We have characterized the hypotensive effect of the RSNO S-nitroso-N-acetylcysteine (SNAC) in normotensive and hypertensive conscious rats. SNAC reduced the medium arterial pressure in a dose-response manner in both normotensive and hypertensive animals. At the same doses (EC(50) of SNAC), SNAC showed a vasodilator effect in normotensive rats more potent and more prolonged than that of sodium nitroprusside (SNP). The hypotensive effect of SNAC was also more potent in methylene blue-treated rats, where the cGMP-dependent pathway had been blockaded. These data indicate that SNAC acts by both cGMP-dependent and cGMP-independent pathways. It was also shown that the thiol N-acetylcysteine (NAC) potentiates the action of SNP in hypertensive rats, pointing to the mediation of thiols in the vasodilator action of SNP in this condition. Such mediation may involve the formation of a more potent thiol complex with the nitroprusside anion or the transfer of NO to NAC, generating SNAC as a primary vasoactive species. The kinetic monitoring of the decomposition reactions of SNAC and SNP showed that both compounds are quite stable under the infusion conditions used. Therefore, their vasodilator action cannot be assigned to their breakdown with release of free NO in solution. As the two compounds are unlikely to cross the plasmalemma of smooth muscle cells, their actions are probably associated with the mediation of endogenous thiols in transnitrosation reactions.
dc.description7
dc.description57-66
dc.languageeng
dc.relationNitric Oxide : Biology And Chemistry / Official Journal Of The Nitric Oxide Society
dc.relationNitric Oxide
dc.rightsfechado
dc.rightsCopyright 2002 Elsevier Science (USA)
dc.sourcePubMed
dc.subjectAcetylcysteine
dc.subjectAnimals
dc.subjectBlood Pressure
dc.subjectCyclic Gmp
dc.subjectDose-response Relationship, Drug
dc.subjectDrug Stability
dc.subjectDrug Synergism
dc.subjectDrug Therapy, Combination
dc.subjectHypertension
dc.subjectMale
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSulfhydryl Compounds
dc.subjectVasodilation
dc.titleCharacterization Of The Hypotensive Effect Of S-nitroso-n-acetylcysteine In Normotensive And Hypertensive Conscious Rats.
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución