G120k-peg, A Human Gh Antagonist, Decreases Gh Signal Transduction In The Liver Of Mice.

dc.creatorThirone, Ana C P
dc.creatorCarvalho, Carla R O
dc.creatorSaad, Mario J A
dc.date2002-Jun
dc.date2015-11-27T12:49:08Z
dc.date2015-11-27T12:49:08Z
dc.date.accessioned2018-03-29T00:56:15Z
dc.date.available2018-03-29T00:56:15Z
dc.identifierMolecular And Cellular Endocrinology. v. 192, n. 1-2, p. 65-74, 2002-Jun.
dc.identifier0303-7207
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/12088868
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195076
dc.identifier12088868
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295309
dc.descriptionAfter receptor binding, growth hormone (GH) induces GH receptors (GHR) dimerization and JAK2 is activated after its association with a dimerized GHR, stimulating the tyrosyl phosphorylation of insulin receptor substrate-1 (IRS-1), IRS-2 and Shc proteins. G120K-PEG, a GH antagonist is produced by a mutation that blocks GH action by preventing the GHR dimerization. This study shows that the inhibitory effect of G120K-PEG was maximal with a GH:G120K-PEG ratio of 1:100, as no increase in JAK2 tyrosyl phosphorylation was observed with this dose of GH. When the dose of GH was increased and with a GH:G120K-PEG ratio of 1:10 some tyrosyl phosphorylation of JAK2 could be observed. Additionally, GH-induced IRS-1, IRS-2 and SHC tyrosyl phosphorylation was inhibited approximately 50% at equimolar concentrations of the antagonist of GH and almost abolished with a GH:G120K-PEG ratio of 1:100. The results clearly show that G120K-PEG inhibits GH signal transduction in mouse liver.
dc.description192
dc.description65-74
dc.languageeng
dc.relationMolecular And Cellular Endocrinology
dc.relationMol. Cell. Endocrinol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAdaptor Proteins, Signal Transducing
dc.subjectAdaptor Proteins, Vesicular Transport
dc.subjectAnimals
dc.subjectDimerization
dc.subjectDose-response Relationship, Drug
dc.subjectHormone Antagonists
dc.subjectHuman Growth Hormone
dc.subjectHumans
dc.subjectInsulin Receptor Substrate Proteins
dc.subjectIntracellular Signaling Peptides And Proteins
dc.subjectJanus Kinase 2
dc.subjectLiver
dc.subjectMale
dc.subjectMice
dc.subjectPhosphoproteins
dc.subjectPhosphorylation
dc.subjectPhosphotyrosine
dc.subjectPolyethylene Glycols
dc.subjectProlactin
dc.subjectProtein Processing, Post-translational
dc.subjectProtein-tyrosine Kinases
dc.subjectProteins
dc.subjectProto-oncogene Proteins
dc.subjectReceptors, Somatotropin
dc.subjectShc Signaling Adaptor Proteins
dc.subjectSignal Transduction
dc.titleG120k-peg, A Human Gh Antagonist, Decreases Gh Signal Transduction In The Liver Of Mice.
dc.typeArtículos de revistas


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