Ca(2+) Transport Into An Intracellular Acidic Compartment Of Candida Parapsilosis.

dc.creatorMilani, G
dc.creatorSchereiber, A Z
dc.creatorVercesi, A E
dc.date2001-Jun
dc.date2015-11-27T12:29:20Z
dc.date2015-11-27T12:29:20Z
dc.date.accessioned2018-03-29T00:56:06Z
dc.date.available2018-03-29T00:56:06Z
dc.identifierFebs Letters. v. 500, n. 1-2, p. 80-4, 2001-Jun.
dc.identifier0014-5793
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/11434931
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195036
dc.identifier11434931
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295269
dc.descriptionIn this report, we study Ca2+ transport in permeabilized Candida parapsilosis spheroplasts prepared by a new technique using lyticase. An intracellular non-mitochondrial Ca2+ uptake pathway, insensitive to orthovanadate and sensitive to the V-H(+)-ATPase inhibitor bafilomycin A(1), nigericin and carbonyl cyanide p-trifluoromethoxyphenylhydrazone was characterized. Acidification of the compartment in which Ca2+ accumulated was followed using the fluorescent dye acridine orange. Acidification was stimulated by the Ca2+ chelator EGTA and inhibited by Ca2+. These results, when added to the observation that Ca2+ induces alkalization of a cellular compartment, provide evidence for the presence of a Ca2+/nH(+) antiporter in the acid compartment membrane. Interestingly, like in acidocalcisomes of trypanosomatids, the antioxidant 3,5-dibutyl-4-hydroxytoluene inhibits the V-H(+)-ATPase. In addition, the antifungal agent ketoconazole promoted a fast alkalization of the acidic compartment. Ketoconazole effects were dose-dependent and occurred in a concentration range close to that attained in the plasma of patients treated with this drug.
dc.description500
dc.description80-4
dc.languageeng
dc.relationFebs Letters
dc.relationFEBS Lett.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAcridine Orange
dc.subjectAdenosine Triphosphate
dc.subjectAmmonium Chloride
dc.subjectAnti-bacterial Agents
dc.subjectAntifungal Agents
dc.subjectAntioxidants
dc.subjectBiological Transport
dc.subjectButylated Hydroxytoluene
dc.subjectCalcium
dc.subjectCandida
dc.subjectKetoconazole
dc.subjectMacrolides
dc.subjectNigericin
dc.subjectSpheroplasts
dc.subjectSubcellular Fractions
dc.subjectVacuoles
dc.titleCa(2+) Transport Into An Intracellular Acidic Compartment Of Candida Parapsilosis.
dc.typeArtículos de revistas


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