Protein Crystal Structure Solution By Fast Incorporation Of Negatively And Positively Charged Anomalous Scatterers.

dc.creatorNagem, R A
dc.creatorDauter, Z
dc.creatorPolikarpov, I
dc.date2001-Jul
dc.date2015-11-27T12:29:19Z
dc.date2015-11-27T12:29:19Z
dc.date.accessioned2018-03-29T00:56:04Z
dc.date.available2018-03-29T00:56:04Z
dc.identifierActa Crystallographica. Section D, Biological Crystallography. v. 57, n. Pt 7, p. 996-1002, 2001-Jul.
dc.identifier0907-4449
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/11418768
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195030
dc.identifier11418768
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295263
dc.descriptionThe preparation of derivatives by the traditional methods of soaking is one of the most time-consuming steps in protein crystal structure solution by X-ray diffraction techniques. The 'quick cryosoaking' procedure for derivatization with halides (monovalent anions) offers the possibility of significantly speeding up this process [Dauter et al. (2000), Acta Cryst. D56, 232-237]. In the present work, an extension of this technique is proposed and the use of two different classes of compounds (monovalent and polyvalent cations) that can be successfully utilized in the quick cryosoaking procedure for the derivatization and phasing of protein crystals is described. This approach has been tested on hen egg-white lysozyme and has been successfully used to solve the structure of a novel trypsin inhibitor. The possibility of using cations in the fast cryosoaking procedure gives additional flexibility in the process of derivatization and increases the chances of success in phase determination. This method can be applied to high-throughput crystallographic projects.
dc.description57
dc.description996-1002
dc.languageeng
dc.relationActa Crystallographica. Section D, Biological Crystallography
dc.relationActa Crystallogr. D Biol. Crystallogr.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectCrystallography, X-ray
dc.subjectModels, Molecular
dc.subjectProtein Conformation
dc.subjectProteins
dc.titleProtein Crystal Structure Solution By Fast Incorporation Of Negatively And Positively Charged Anomalous Scatterers.
dc.typeArtículos de revistas


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