Expression And Distribution Of Nos1 And Nos3 In The Myocardium Of Angiotensin Ii-infused Rats.

dc.creatorTambascia, R C
dc.creatorFonseca, P M
dc.creatorCorat, P D
dc.creatorMoreno, H
dc.creatorSaad, M J
dc.creatorFranchini, K G
dc.date2001-Jun
dc.date2015-11-27T12:29:19Z
dc.date2015-11-27T12:29:19Z
dc.date.accessioned2018-03-29T00:56:03Z
dc.date.available2018-03-29T00:56:03Z
dc.identifierHypertension. v. 37, n. 6, p. 1423-8, 2001-Jun.
dc.identifier1524-4563
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/11408389
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195025
dc.identifier11408389
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295258
dc.descriptionStudies have indicated a complex functional interaction between angiotensin (Ang) II and NO in the heart. The purpose of the present study was to examine the protein expression and tissue distribution of NO synthases 1 (NOS1) and 3 (NOS3) in the myocardium of rats that underwent continuous infusion of Ang II at 2 different rates (10 and 40 ng. kg(-1). min(-1)) for 6 days. Mean arterial pressure increased by approximately 15 mm Hg in rats infused with Ang II at 40 ng. kg(-1). min(-1), but it remained close to the values observed in saline-infused rats ( approximately 110 mm Hg) when Ang II was infused at 10 ng. kg(-1). min(-1). The protein expression of a 160-kDa NOS1 and a 135-kDa NOS3 were found to increase ( approximately 200%) in the myocardium of rats infused with both subpressor and pressor doses of Ang II. Immunohistochemistry studies showed that NOS1 and NOS3 are differentially expressed in myocardial cells. NOS1 was detected in cardiac myocytes and in smooth muscle cells of small and large coronary arteries, whereas NOS3 was detected in the endothelium and in perivascular and interstitial tissues, but NOS3 was not detected in cardiac or smooth muscle cells. Ang II infusion enhanced the tissue immunoreactivity of both isoforms in their specific locations but did not change the distribution throughout the myocardium. Myocardium staining with anti-angiotensin type 1 (AT(1)) receptor antibody indicated that AT(1) receptor is expressed in cardiac myocytes, coronary smooth muscle cells, and interstitial and perivascular tissues. Ang II infusion did not change the protein expression and distribution of AT(1) receptor in the myocardium. These results indicate that long-term increases in the circulating levels of Ang II modulate the protein expression of NOS1 and NOS3 and, consequently, the function of the local myocardial NO system.
dc.description37
dc.description1423-8
dc.languageeng
dc.relationHypertension
dc.relationHypertension
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAngiotensin Ii
dc.subjectAnimals
dc.subjectBlood Pressure
dc.subjectCoronary Vessels
dc.subjectEndothelium, Vascular
dc.subjectHeart Ventricles
dc.subjectInfusions, Intravenous
dc.subjectMale
dc.subjectMuscle, Smooth, Vascular
dc.subjectMyocardium
dc.subjectNitric Oxide Synthase
dc.subjectNitric Oxide Synthase Type I
dc.subjectNitric Oxide Synthase Type Iii
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReceptor, Angiotensin, Type 1
dc.subjectReceptor, Angiotensin, Type 2
dc.subjectReceptors, Angiotensin
dc.subjectVasoconstrictor Agents
dc.titleExpression And Distribution Of Nos1 And Nos3 In The Myocardium Of Angiotensin Ii-infused Rats.
dc.typeArtículos de revistas


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