Eae-negative Attaching And Effacing Escherichia Coli From Piglets With Diarrhea.

dc.creatorPenteado, A S
dc.creatorAidar, L
dc.creatorPestana de Castro, A F
dc.creatorYamada, A
dc.creatorAndrade, J R
dc.creatorBlanco, J
dc.creatorBlanco, M
dc.creatorBlanco, J E
dc.date
dc.date2015-11-27T12:29:13Z
dc.date2015-11-27T12:29:13Z
dc.date.accessioned2018-03-29T00:55:51Z
dc.date.available2018-03-29T00:55:51Z
dc.identifierResearch In Microbiology. v. 152, n. 1, p. 75-81
dc.identifier0923-2508
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/11281328
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194973
dc.identifier11281328
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295206
dc.descriptionOne hundred and ninety strains of Escherichia coli that were isolated from pigs with diarrhea in the state of São Paulo, Brazil, and that were negative for enterotoxins and cytotoxins were investigated. Strains which adhered to HeLa cells were examined for fluorescence actin staining (FAS), the ability to induce attaching and effacing (A/E) lesions on HEp-2 cells detectable by transmission electron microscopy and the presence of eae gene sequences detected by PCR. Intimin production was detected by western blot and serogrouping was performed. Forty-seven isolates adhered to HeLa cells in several patterns, but none adhered in a localized adherence pattern. However, seven of the 47 adherent strains were positive for the FAS reaction, although the reactions were usually weak or atypical. One FAS-negative and three FAS-positive strains, which were examined for their ability to induce A/E lesions, were all positive. Subsequently, testing of these strains for the eae gene showed that they all lacked this gene. These findings, along with earlier reports of eae-negative A/E E. coli, suggest that higher quantities of E. coli in this category might be detected if more reliance were placed on phenotypic tests rather than on gene detection tests alone.
dc.description152
dc.description75-81
dc.languageeng
dc.relationResearch In Microbiology
dc.relationRes. Microbiol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAdhesins, Bacterial
dc.subjectAnimals
dc.subjectBacterial Adhesion
dc.subjectBacterial Outer Membrane Proteins
dc.subjectBlotting, Western
dc.subjectCarrier Proteins
dc.subjectCercopithecus Aethiops
dc.subjectDiarrhea
dc.subjectEscherichia Coli
dc.subjectEscherichia Coli Infections
dc.subjectEscherichia Coli Proteins
dc.subjectGenotype
dc.subjectHela Cells
dc.subjectHumans
dc.subjectMicroscopy, Electron
dc.subjectPhenotype
dc.subjectPolymerase Chain Reaction
dc.subjectShiga Toxins
dc.subjectSwine
dc.subjectSwine Diseases
dc.subjectVero Cells
dc.titleEae-negative Attaching And Effacing Escherichia Coli From Piglets With Diarrhea.
dc.typeArtículos de revistas


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