Apoptosis Of Sensory Neurons And Satellite Cells After Sciatic Nerve Transection In C57bl/6j Mice.

dc.creatorOliveira, A L
dc.date2001-Mar
dc.date2015-11-27T12:29:09Z
dc.date2015-11-27T12:29:09Z
dc.date.accessioned2018-03-29T00:55:42Z
dc.date.available2018-03-29T00:55:42Z
dc.identifierBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 34, n. 3, p. 375-80, 2001-Mar.
dc.identifier0100-879X
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/11262589
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194932
dc.identifier11262589
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295165
dc.descriptionThe rate of axonal regeneration, after sciatic nerve lesion in adult C57BL/6J mice, is reduced when compared to other isogenic strains. It was observed that such low regeneration was not due just to a delay, since neuronal death was observed. Two general mechanisms of cell death, apoptosis and necrosis, may be involved. By using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) technique, we demonstrated that a large number of sensory neurons, as well as satellite cells found in the dorsal root ganglia, were intensely labeled, thus indicating that apoptotic mechanisms were involved in the death process. Although almost no labeled neurons or satellite cells were observed one week after transection, a more than ten-fold increase in TUNEL labeling was detected after two weeks. The results obtained with the C57BL/6J strain were compared with those of the A/J strain, which has a much higher peripheral nerve regeneration potential. In A/J mice, almost no labeling of sensory neurons or satellite cells was observed after one or two weeks, indicating the absence of neuronal loss. Our data confirm previous observations that approximately 40% of C57BL/6J sensory neurons die after sciatic nerve transection, and indicate that apoptotic events are involved. Also, our observations reinforce the hypothesis that the low rate of axonal regeneration occurring in C57BL/6J mice may be the result of a mismatch in the timing of the neurons need for neurotrophic substances, and production of the latter by non-neuronal cells in the distal stump.
dc.description34
dc.description375-80
dc.languageeng
dc.relationBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]
dc.relationBraz. J. Med. Biol. Res.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectIn Situ Nick-end Labeling
dc.subjectMale
dc.subjectMice
dc.subjectMice, Inbred C57bl
dc.subjectMuscle Fibers, Skeletal
dc.subjectMuscle, Skeletal
dc.subjectNerve Fibers
dc.subjectNerve Regeneration
dc.subjectNeurons, Afferent
dc.subjectSciatic Nerve
dc.titleApoptosis Of Sensory Neurons And Satellite Cells After Sciatic Nerve Transection In C57bl/6j Mice.
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución