Tissue-specific Regulation Of Early Steps In Insulin Action In Septic Rats.

dc.creatorNunes, A L
dc.creatorCarvalheira, J B
dc.creatorCarvalho, C R
dc.creatorBrenelli, S L
dc.creatorSaad, M J
dc.date2001-Sep
dc.date2015-11-27T12:29:02Z
dc.date2015-11-27T12:29:02Z
dc.date.accessioned2018-03-29T00:55:28Z
dc.date.available2018-03-29T00:55:28Z
dc.identifierLife Sciences. v. 69, n. 18, p. 2103-12, 2001-Sep.
dc.identifier0024-3205
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/11669454
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194872
dc.identifier11669454
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295105
dc.descriptionSepsis is known to induce insulin resistance, but the exact molecular mechanism involved is unknown. In the present study we have examined the levels and phosphorylation state of the insulin receptor and of insulin receptor substrate 1 (IRS-1), as well as the association between IRS-1 and phosphatidylinositol 3-kinase (PI 3-kinase) in the liver and muscle of septic rats by immunoprecipitation and immunoblotting with anti-insulin receptor, anti-IRS-1, anti-PI 3-kinase and anti-phosphotyrosine antibodies. There were no changes in the insulin receptor concentration and phosphorylation levels in the liver and muscle of septic rats. IRS-1 protein levels were decreased by 40+/-3% (p < 0.01) in muscle but not in liver of septic rats. In samples previously immunoprecipitated with anti-IRS-1 antibody and blotted with antiphosphotyrosine antibody, the insulin-stimulated IRS-1 phosphorylation levels in the muscle of septic rats decreased by 38+/-5% (p < 0.01) and insulin-stimulated IRS-1 association with PI 3-kinase decreased by 44+/-7% in muscle (p < 0.01) but no changes were seen in liver. These data suggest that there is a tissue-specific regulation of early steps of insulin signal transduction in septic rats, and the changes observed in muscle may have a role in the insulin resistance of these animals.
dc.description69
dc.description2103-12
dc.languageeng
dc.relationLife Sciences
dc.relationLife Sci.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectInsulin
dc.subjectInsulin Receptor Substrate Proteins
dc.subjectInsulin Resistance
dc.subjectMale
dc.subjectOrgan Specificity
dc.subjectPhosphoproteins
dc.subjectPhosphorylation
dc.subjectRats
dc.subjectReceptor, Insulin
dc.subjectSepsis
dc.subjectSignal Transduction
dc.titleTissue-specific Regulation Of Early Steps In Insulin Action In Septic Rats.
dc.typeArtículos de revistas


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