Blood Group Genotyping In A Population Of Highly Diverse Ancestry.

dc.creatorPellegrino, J
dc.creatorCastilho, L
dc.creatorRios, M
dc.creatorDe Souza, C A
dc.date2001
dc.date2015-11-27T12:28:51Z
dc.date2015-11-27T12:28:51Z
dc.date.accessioned2018-03-29T00:55:08Z
dc.date.available2018-03-29T00:55:08Z
dc.identifierJournal Of Clinical Laboratory Analysis. v. 15, n. 1, p. 8-13, 2001.
dc.identifier0887-8013
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/11170227
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194789
dc.identifier11170227
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295022
dc.descriptionAccurate phenotyping of red blood cells (RBCs) can be difficult in transfusion-dependent patients such as those with thalassemia and sickle cell anemia because of the presence of previously transfused RBCs in the patient's circulation. Recently, the molecular basis associated with the expression of many blood group antigens was established. This allowed the development of a plethora of polymerase chain reaction (PCR)-based tests for identification of the blood group antigens by testing DNA. The new technologies complement phenotyping and overcome some of the limitations of hemagglutination assays. These molecular assays were developed on the basis of DNA sequences of individuals of Caucasian ancestry. The present study addresses the concern that these genotyping assays may not be applicable to populations of highly diverse ancestry because of variability in intronic regions or because of unrecognized alleles. We determined both phenotype and genotype for RH D, K 1/K 2, JK A/JK B, FY A/ FY B-GATA in 250 normal blood donors using PCR. Phenotype and genotype results agreed in 100% of the cases, indicating that molecular genotyping protocols can be effectively applied to populations with a highly diverse genetic background. However, genotyping for Duffy antigens provided information that could not be obtained by phenotyping. Essentially, 30.5 % of the donors with the FY B gene typed as Fy(b-) because of mutations in the GATA box. This information is very useful for the management of transfusion dependent patients.
dc.description15
dc.description8-13
dc.languageeng
dc.relationJournal Of Clinical Laboratory Analysis
dc.relationJ. Clin. Lab. Anal.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectBlood Donors
dc.subjectBlood Group Antigens
dc.subjectBlood Transfusion
dc.subjectDuffy Blood-group System
dc.subjectElectrophoresis, Polyacrylamide Gel
dc.subjectEuropean Continental Ancestry Group
dc.subjectExons
dc.subjectGenotype
dc.subjectHumans
dc.subjectIntrons
dc.subjectKell Blood-group System
dc.subjectKidd Blood-group System
dc.subjectPhenotype
dc.subjectPolymerase Chain Reaction
dc.subjectPolymorphism, Restriction Fragment Length
dc.subjectRh-hr Blood-group System
dc.titleBlood Group Genotyping In A Population Of Highly Diverse Ancestry.
dc.typeArtículos de revistas


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