dc.creatorLôbo de Araújo, A
dc.creatorKamiguti, A
dc.creatorBon, C
dc.date
dc.date2015-11-27T12:28:48Z
dc.date2015-11-27T12:28:48Z
dc.date.accessioned2018-03-29T00:55:06Z
dc.date.available2018-03-29T00:55:06Z
dc.identifierToxicon : Official Journal Of The International Society On Toxinology. v. 39, n. 2-3, p. 371-5
dc.identifier0041-0101
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/10978756
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194779
dc.identifier10978756
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295012
dc.descriptionBothrops lanceolatus venom contains caseinolytic, phospholipase, esterase and haemorrhagic activities. We have investigated the coagulant and anticoagulant actions of B. lanceolatus venom on human citrated plasma and on purified plasma components. Although B. lanceolatus venom up to 50 microg/ml was unable to clot citrated plasma, at concentrations > or = 5 microg/ml the venom dose-dependently clotted purified human fibrinogen, indicating the presence of a thrombin-like enzyme. Human plasma (final concentration > or = 12.5%) dose-dependently inhibited the venom-induced fibrinogen clotting. This finding suggested that endogenous plasma protease inhibitors can affect the venom's action on fibrinogen. To investigate this possibility, B. lanceolatus venom was incubated with different plasma protease inhibitors and the activity on fibrinogen tested. alpha(2)-Macroglobulin and alpha(1)-antitrypsin did not interfere with the coagulant activity of the venom whereas the antithrombin-III/heparin complex partially inhibited this activity. A non-toxic, acidic phospholipase A(2) purified from B. lanceolatus venom prolonged the activated partial thromboplastin time in human plasma from 39.7+/-0.5 s (control with saline) to 60.2+/-0.9 s with 50 microg of PLA(2) (p<0.001), suggesting an anticoagulant activity associated with this enzyme. This anticoagulant activity may account for some of the effects of the venom on blood coagulation.
dc.description39
dc.description371-5
dc.languageeng
dc.relationToxicon : Official Journal Of The International Society On Toxinology
dc.relationToxicon
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBlood Coagulation
dc.subjectBothrops
dc.subjectCrotalid Venoms
dc.subjectFibrinogen
dc.subjectHumans
dc.subjectPhospholipases A
dc.titleCoagulant And Anticoagulant Activities Of Bothrops Lanceolatus (fer De Lance) Venom.
dc.typeArtículos de revistas


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