dc.creatorSilva, F R
dc.creatorSantana, C C
dc.date2000
dc.date2015-11-27T12:23:01Z
dc.date2015-11-27T12:23:01Z
dc.date.accessioned2018-03-29T00:55:03Z
dc.date.available2018-03-29T00:55:03Z
dc.identifierApplied Biochemistry And Biotechnology. v. 84-86, p. 1063-78, 2000.
dc.identifier0273-2289
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/10849858
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194764
dc.identifier10849858
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294997
dc.descriptionThe use of adsorption columns packed with ion-exchange resins for recovering, concentrating and purifying proteins is now widespread. The present work consists of a study on the dynamic behavior of adsorption columns that uses two kinds of adsorbents: a cationic and an anionic resin. A frontal analysis of the columns was performed with experimental data obtained from Fructozyme, a mixture of inulinase enzymes. The parameters of a Langmuir type of isotherm and adsorption kinetics were obtained from experimental tests in a batch system. A numerical technique based on orthogonal collocation and a fourth-order Runge-Kutta method was coupled with a nonlinear optimization method to predict the coefficients of the rate equations, which are fundamental for scale-up purposes.
dc.description84-86
dc.description1063-78
dc.languageeng
dc.relationApplied Biochemistry And Biotechnology
dc.relationAppl. Biochem. Biotechnol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAdsorption
dc.subjectAspergillus
dc.subjectAspergillus Niger
dc.subjectChromatography, Ion Exchange
dc.subjectChrysosporium
dc.subjectGlycoside Hydrolases
dc.subjectIon Exchange Resins
dc.subjectKinetics
dc.subjectPenicillium
dc.subjectSepharose
dc.titleAdsorption Of Inulinases In Ion-exchange Columns.
dc.typeArtículos de revistas


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