Characterization Of The Insulin-signaling Pathway In Lacrimal And Salivary Glands Of Rats.

dc.creatorRocha, E M
dc.creatorde M Lima, M H
dc.creatorCarvalho, C R
dc.creatorSaad, M J
dc.creatorVelloso, L A
dc.date2000-Nov
dc.date2015-11-27T12:22:51Z
dc.date2015-11-27T12:22:51Z
dc.date.accessioned2018-03-29T00:54:41Z
dc.date.available2018-03-29T00:54:41Z
dc.identifierCurrent Eye Research. v. 21, n. 5, p. 833-42, 2000-Nov.
dc.identifier0271-3683
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/11262604
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194672
dc.identifier11262604
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294905
dc.descriptionInsulin has been acknowledged as a mediator of several physiological events in lacrimal and salivary glands. We investigated the presence of insulin receptors and of insulin-induced autophosphorylation of the insulin receptor and activation of elements involved in the early steps of insulin signaling in lacrimal and salivary glands of rats. Lacrimal and salivary glands of Wistar rats were removed and processed for immunohistochemistry using anti-insulin receptor and anti-IGF-1 receptor antibodies. The activation of insulin receptors following insulin treatment, and the involvement of insulin receptor substrates-1 and -2, Shc, JAK-2 and STAT-1, were analyzed by immunoprecipitation, followed by SDS-PAGE and immunoblotting of rat lacrimal and salivary glands after exposure to insulin. Insulin and IGF-1 receptors were present in rat lacrimal and salivary glands and were located predominantly in the cytoplasm and plasma membrane. Functional studies demonstrated that insulin induced a dose-dependent phosphorylation of the insulin receptor, IGF-1R, insulin receptor substrates-1 and -2, Shc, and STAT-1. In rats with streptozotocin-induced diabetes mellitus there was a significant reduction in insulin-induced insulin receptor and STAT-1 phosphorylation in the lacrimal gland but not in the salivary gland; there was no influence on Shc phosphorylation in either tissue. The present results indicate that insulin and IGF-1 receptors are expressed in lacrimal and salivary glands, and that insulin can induce the phosphorylation of its receptor and activate elements involved in the early steps of insulin signaling in both tissues.
dc.description21
dc.description833-42
dc.languageeng
dc.relationCurrent Eye Research
dc.relationCurr. Eye Res.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAdaptor Proteins, Signal Transducing
dc.subjectAdaptor Proteins, Vesicular Transport
dc.subjectAnimals
dc.subjectDna-binding Proteins
dc.subjectDiabetes Mellitus, Experimental
dc.subjectDose-response Relationship, Drug
dc.subjectElectrophoresis, Polyacrylamide Gel
dc.subjectImmunoblotting
dc.subjectInsulin
dc.subjectInsulin Receptor Substrate Proteins
dc.subjectIntracellular Signaling Peptides And Proteins
dc.subjectJanus Kinase 2
dc.subjectLacrimal Apparatus
dc.subjectMale
dc.subjectPhosphoproteins
dc.subjectPhosphorylation
dc.subjectPrecipitin Tests
dc.subjectProtein-tyrosine Kinases
dc.subjectProteins
dc.subjectProto-oncogene Proteins
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReceptor, Igf Type 1
dc.subjectReceptor, Insulin
dc.subjectStat1 Transcription Factor
dc.subjectSalivary Glands
dc.subjectShc Signaling Adaptor Proteins
dc.subjectSignal Transduction
dc.subjectTrans-activators
dc.subjectTyrosine
dc.titleCharacterization Of The Insulin-signaling Pathway In Lacrimal And Salivary Glands Of Rats.
dc.typeArtículos de revistas


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