Restriction Enzyme Analysis Of Dna Methylation In Condensed" Chromatin Of Ha-ras-transformed Nih 3t3 Cells."

dc.creatorMello, M L
dc.creatorChambers, A F
dc.creatorVidal, B C
dc.creatorPlanding, W
dc.creatorSchenck, U
dc.date2000
dc.date2015-11-27T12:22:51Z
dc.date2015-11-27T12:22:51Z
dc.date.accessioned2018-03-29T00:54:41Z
dc.date.available2018-03-29T00:54:41Z
dc.identifierAnalytical Cellular Pathology : The Journal Of The European Society For Analytical Cellular Pathology. v. 20, n. 4, p. 163-71, 2000.
dc.identifier0921-8912
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/11205319
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194669
dc.identifier11205319
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294902
dc.descriptionIncreased amounts of chromatin condensation (i.e., localized areas of high DNA density, or chromatin higher order packing state) have been described in NIH 3T3 cells transformed with the Ha-ras oncogene. The structural basis for this oncogene-mediated alteration in nuclear organization is unknown. Since DNA methylation is likely to be involved in regulating the nucleosomal level of DNA packaging, we studied the role of DNA methylation in higher-order chromatin organization induced by Ha-ras. CpG-methylated DNA content was estimated in condensed chromatin of Ha-ras-transformed NIH 3T3 cell lines which differ in ras expression and ras-induced metastatic ability but present approximately the same values of condensed chromatin areas. The question posed was that if DNA methylation were involved with the chromatin higher-order organization induced by Ha-ras in these cell lines, the methylated DNA density in the condensed chromatin would also be the same. The DNA evaluation was performed by video image analysis in Feulgen-stained cells previously subjected to treatment with Msp I and Hpa II restriction enzymes, which distinguish between methylated and non-methylated DNA. The amount of methylated CpG sequences not digested by Hpa II in condensed chromatin regions was found to vary in the studied ras-transformed cell lines. DNA CpG methylation status is thus suggested not to be involved with the higher order chromatin condensation induced by ras transformation in the mentioned NIH 3T3 cell lines.
dc.description20
dc.description163-71
dc.languageeng
dc.relationAnalytical Cellular Pathology : The Journal Of The European Society For Analytical Cellular Pathology
dc.relationAnal Cell Pathol
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subject3t3 Cells
dc.subjectAnimals
dc.subjectCell Line, Transformed
dc.subjectChromatin
dc.subjectColoring Agents
dc.subjectDna
dc.subjectDna Methylation
dc.subjectDna-cytosine Methylases
dc.subjectDeoxyribonuclease Hpaii
dc.subjectGenes, Ras
dc.subjectImage Processing, Computer-assisted
dc.subjectMice
dc.subjectMicroscopy, Video
dc.subjectRosaniline Dyes
dc.titleRestriction Enzyme Analysis Of Dna Methylation In Condensed" Chromatin Of Ha-ras-transformed Nih 3t3 Cells."
dc.typeArtículos de revistas


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