Brasil
| Artículos de revistas
Cytotoxicity Of Prodigiosin And Benznidazole On V79 Cells.
| dc.creator | da Silva Melo, P | |
| dc.creator | Durán, N | |
| dc.creator | Haun, M | |
| dc.date | 2000-Aug | |
| dc.date | 2015-11-27T12:22:40Z | |
| dc.date | 2015-11-27T12:22:40Z | |
| dc.date.accessioned | 2018-03-29T00:54:21Z | |
| dc.date.available | 2018-03-29T00:54:21Z | |
| dc.identifier | Toxicology Letters. v. 116, n. 3, p. 237-42, 2000-Aug. | |
| dc.identifier | 0378-4274 | |
| dc.identifier | ||
| dc.identifier | http://www.ncbi.nlm.nih.gov/pubmed/10996486 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/194586 | |
| dc.identifier | 10996486 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1294819 | |
| dc.description | The cytotoxicity of prodigiosin, an antibiotic and potential trypanocide produced by Serratia marcescens, and Benznidazole, a trypanocidal drug, were assayed on V79 fibroblast cell line. Three independent endpoints for cytotoxicity were evaluated; namely, the nucleic acid content (NAC), MTT reduction and neutral red uptake (NRU). IC(50) values of 1-20 microM were obtained for prodigiosin in the NRU, MTT and NAC tests. Prodigiosin had greater trypanocidal activity (IC(50)=5 microM) than Nifurtimox (IC(50)=150 microM) a known trypanocide drug used in Chagas' disease therapy. Benznidazole was less toxic (IC(50)=2000 microM) than prodigiosin (IC(50)=1-20 microM) in V79 cells based on the MTT and NAC assays. Benznidazole stimulated the NRU until 2 mM. Indeed, the cell viability measured with the NRU was higher at all concentrations of benznidazole tested than that measured by MTT reduction and NAC assays. | |
| dc.description | 116 | |
| dc.description | 237-42 | |
| dc.language | eng | |
| dc.relation | Toxicology Letters | |
| dc.relation | Toxicol. Lett. | |
| dc.rights | fechado | |
| dc.rights | ||
| dc.source | PubMed | |
| dc.subject | Animals | |
| dc.subject | Cell Survival | |
| dc.subject | Cells, Cultured | |
| dc.subject | Cricetinae | |
| dc.subject | Cricetulus | |
| dc.subject | Dose-response Relationship, Drug | |
| dc.subject | Nitroimidazoles | |
| dc.subject | Prodigiosin | |
| dc.subject | Trypanocidal Agents | |
| dc.title | Cytotoxicity Of Prodigiosin And Benznidazole On V79 Cells. | |
| dc.type | Artículos de revistas |