Inhibition Of Mitochondrial Permeability Transition By Low Ph Is Associated With Less Extensive Membrane Protein Thiol Oxidation.

dc.creatorTeixeira, B M
dc.creatorKowaltowski, A J
dc.creatorCastilho, R F
dc.creatorVercesi, A E
dc.date1999-Dec
dc.date2015-11-27T12:19:49Z
dc.date2015-11-27T12:19:49Z
dc.date.accessioned2018-03-29T00:54:00Z
dc.date.available2018-03-29T00:54:00Z
dc.identifierBioscience Reports. v. 19, n. 6, p. 525-33, 1999-Dec.
dc.identifier0144-8463
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/10841269
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194492
dc.identifier10841269
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294725
dc.descriptionCa2+ and inorganic phosphate-induced mitochondrial swelling and membrane protein thiol oxidation, which are associated with mitochondrial permeability transition, are inhibited by progressively decreasing the incubation medium pH between 7.2 and 6.0. Nevertheless, the detection of mitochondrial H2O2 production under these conditions is increased. Permeability transition induced by phenylarsine oxide, which promotes membrane protein thiol cross-linkage in a process independent of Ca2+ or reactive oxygen species, is also strongly inhibited in acidic incubation media. In addition, we observed that the decreased protein thiol reactivity with phenylarsine oxide or phenylarsine oxide-induced swelling at pH 6.0 is reversed by diethyl pyrocarbonate, in a hydroxylamine-sensitive manner. These results provide evidence that the inhibition of mitrochondrial permeability transition observed at lower incubation medium pH is mediated by a decrease in membrane protein thiol reactivity, related to the protonation of protein histidyl residues.
dc.description19
dc.description525-33
dc.languageeng
dc.relationBioscience Reports
dc.relationBiosci. Rep.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAntimycin A
dc.subjectArsenicals
dc.subjectCalcimycin
dc.subjectCalcium
dc.subjectCarbonyl Cyanide P-trifluoromethoxyphenylhydrazone
dc.subjectDiethyl Pyrocarbonate
dc.subjectHydrogen-ion Concentration
dc.subjectHydroxylamine
dc.subjectIntracellular Membranes
dc.subjectIonophores
dc.subjectMembrane Proteins
dc.subjectMitochondria, Liver
dc.subjectOxidation-reduction
dc.subjectPermeability
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSerum Albumin, Bovine
dc.subjectSulfhydryl Compounds
dc.titleInhibition Of Mitochondrial Permeability Transition By Low Ph Is Associated With Less Extensive Membrane Protein Thiol Oxidation.
dc.typeArtículos de revistas


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