Alteration In Expression Of Mmp-1 And Mmp-2 But Not Timp-1 And Timp-2 In Hereditary Gingival Fibromatosis Is Mediated By Tgf-beta 1 Autocrine Stimulation.

dc.creatorColetta, R D
dc.creatorAlmeida, O P
dc.creatorReynolds, M A
dc.creatorSauk, J J
dc.date1999-Nov
dc.date2015-11-27T12:19:48Z
dc.date2015-11-27T12:19:48Z
dc.date.accessioned2018-03-29T00:53:56Z
dc.date.available2018-03-29T00:53:56Z
dc.identifierJournal Of Periodontal Research. v. 34, n. 8, p. 457-63, 1999-Nov.
dc.identifier0022-3484
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/10697802
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194476
dc.identifier10697802
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294709
dc.descriptionHereditary gingival fibromatosis (HGF) is characterized by an excess accumulation of extracellular matrix (ECM) resulting in a generalized and fibrotic enlargement of the gingiva. To investigate some of the regulatory features of this condition, gingival fibroblasts from normal gingiva (NG) and HGF were examined for the expression and production of matrix metalloproteinases (MMPs) and their inhibitors, tissue matrix metalloproteinases inhibitor (TIMPs). Our results, obtained from 2 different assays, semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and enzymography, clearly demonstrated that the expression and production of MMP-1 and MMP-2 was significantly lower in fibroblasts from HGF than from NG. Interestingly, TIMP-1 and TIMP-2 expression from NG cells was shown to be slightly higher to those from HGF. Addition of antibodies against transforming growth factor-beta 1 (TGF-beta 1), which is produced in greater amounts by HGF fibroblasts, resulted in a slight increase in MMP-1 and a decrease in MMP-2 expression, whereas TIMP-1 and TIMP-2 expressions were unaffected. These patterns of expression and production suggest that enhanced TGF-beta 1 production reduce the proteolytic activities of HGF fibroblasts, which favor the accumulation of ECM.
dc.description34
dc.description457-63
dc.languageeng
dc.relationJournal Of Periodontal Research
dc.relationJ. Periodont. Res.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectBase Sequence
dc.subjectCells, Cultured
dc.subjectDna Primers
dc.subjectFibroblasts
dc.subjectFibromatosis, Gingival
dc.subjectGene Expression Regulation, Enzymologic
dc.subjectGingiva
dc.subjectHumans
dc.subjectMatrix Metalloproteinase 1
dc.subjectMatrix Metalloproteinase 2
dc.subjectMolecular Sequence Data
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectTissue Inhibitor Of Metalloproteinase-1
dc.subjectTissue Inhibitor Of Metalloproteinase-2
dc.subjectTransforming Growth Factor Beta
dc.titleAlteration In Expression Of Mmp-1 And Mmp-2 But Not Timp-1 And Timp-2 In Hereditary Gingival Fibromatosis Is Mediated By Tgf-beta 1 Autocrine Stimulation.
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución