Plant Uncoupling Mitochondrial Protein Activity In Mitochondria Isolated From Tomatoes At Different Stages Of Ripening.

dc.creatorCosta, A D
dc.creatorNantes, I L
dc.creatorJezek, P
dc.creatorLeite, A
dc.creatorArruda, P
dc.creatorVercesi, A E
dc.date1999-Oct
dc.date2015-11-27T12:19:46Z
dc.date2015-11-27T12:19:46Z
dc.date.accessioned2018-03-29T00:53:54Z
dc.date.available2018-03-29T00:53:54Z
dc.identifierJournal Of Bioenergetics And Biomembranes. v. 31, n. 5, p. 527-33, 1999-Oct.
dc.identifier0145-479X
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/10653480
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194464
dc.identifier10653480
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294697
dc.descriptionIn the present study we have observed a higher state of coupling in respiring mitochondria isolated from green as compared to red tomatoes (Lycopersicon esculentum, Mill.). Green tomato mitochondria produced a membrane potential (deltapsi) high enough to phosphorylate ADP, whereas in red tomato mitochondria, BSA and ATP were required to restore deltapsi to the level of that obtained with green tomato mitochondria. This supports the notion that such uncoupling in red tomato mitochondria is mediated by a plant uncoupling mitochondrial protein (PUMP; cf. Vercesi et al., 1995). Nevertheless, mitochondria from both green and red tomatoes exhibited an ATP-sensitive linoleic acid (LA)-induced deltapsi decrease providing evidence that PUMP is also present in green tomatoes. Indeed, proteoliposomes containing reconstituted green or red tomato PUMP showed LA uniport and LA-induced H+ transport. It is suggested that the higher concentration of free fatty acids (PUMP substrates) in red tomatoes could explain the lower coupling state in mitochondria isolated from these fruits.
dc.description31
dc.description527-33
dc.languageeng
dc.relationJournal Of Bioenergetics And Biomembranes
dc.relationJ. Bioenerg. Biomembr.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCarrier Proteins
dc.subjectFatty Acids
dc.subjectIntracellular Membranes
dc.subjectIon Channels
dc.subjectLycopersicon Esculentum
dc.subjectMembrane Proteins
dc.subjectMitochondria
dc.subjectMitochondrial Proteins
dc.subjectProteolipids
dc.subjectProtons
dc.subjectUncoupling Agents
dc.titlePlant Uncoupling Mitochondrial Protein Activity In Mitochondria Isolated From Tomatoes At Different Stages Of Ripening.
dc.typeArtículos de revistas


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