Xanthine Degradation And Related Enzyme Activities In Leaves And Fruits Of Two Coffea Species Differing In Caffeine Catabolism.

dc.creatorVitória, A P
dc.creatorMazzafera, P
dc.date1999-May
dc.date2015-11-27T12:19:44Z
dc.date2015-11-27T12:19:44Z
dc.date.accessioned2018-03-29T00:53:49Z
dc.date.available2018-03-29T00:53:49Z
dc.identifierJournal Of Agricultural And Food Chemistry. v. 47, n. 5, p. 1851-5, 1999-May.
dc.identifier0021-8561
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/10552461
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194442
dc.identifier10552461
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294675
dc.descriptionThe degradation of xanthine was studied in young and aged leaves and in immature and mature fruits of Coffea arabica and Coffea dewevrei, which differ with respect to caffeine catabolism. Radioisotope feeding experiments showed that leaves degraded xanthine more readily than fruits but that mature fruits and aged leaves were less efficient than younger tissues. In all cases, a significant part of the recovered radioactivity was in the ureides. Xanthine dehydrogenase was characterized as the enzyme responsible for xanthine degradation, and its activity and that of uricase were consistent with the results obtained in the radioisotope feeding experiments. Activities of allantoinase and allantoate amidohydrolase could not be detected. Considerable levels of endogenous allantoin and allantoic acid were found in fruits and leaves. Therefore, ureide accumulation might be a consequence of low enzyme activity. There was no positive correlation between urease activity and the data from the radioisotope feeding experiments.
dc.description47
dc.description1851-5
dc.languageeng
dc.relationJournal Of Agricultural And Food Chemistry
dc.relationJ. Agric. Food Chem.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectCaffeine
dc.subjectCoffee
dc.subjectPlant Leaves
dc.subjectSeeds
dc.subjectSpecies Specificity
dc.subjectUrate Oxidase
dc.subjectXanthine
dc.subjectXanthine Dehydrogenase
dc.titleXanthine Degradation And Related Enzyme Activities In Leaves And Fruits Of Two Coffea Species Differing In Caffeine Catabolism.
dc.typeArtículos de revistas


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