Molecular Characterization Of Brazilian Avian Pneumovirus Isolates: Comparison Between Immunochemiluminescent Southern Blot And Nested Pcr.

dc.creatorDani, M A
dc.creatorDurigon, E L
dc.creatorArns, C W
dc.date1999-May
dc.date2015-11-27T12:19:37Z
dc.date2015-11-27T12:19:37Z
dc.date.accessioned2018-03-29T00:53:33Z
dc.date.available2018-03-29T00:53:33Z
dc.identifierJournal Of Virological Methods. v. 79, n. 2, p. 237-41, 1999-May.
dc.identifier0166-0934
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/10381093
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194373
dc.identifier10381093
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294606
dc.descriptionAvian pneumovirus (APV) causes acute respiratory tract infection both in turkeys (turkey rhinotracheitis) and chickens (swollen head syndrome (SHS)) with sudden onset and rapid spread through the flocks. In this study, an immunochemiluminescent Southern blot RT-PCR assay was employed to detect a F gene transcript of the APV in two European turkey isolates and two Brazilian chicken isolates. Limiting dilution PCR was carried out to compare the sensitivity of immunochemiluminescent Southern blot assay and nested PCR assay (nPCR). The sensitivity and specificity of immunochemiluminescent Southern blot RT-PCR assay were comparable to that of nPCR, and at least 100 fold more sensitive than a single PCR amplification. Sequence analysis of the 175 bp product of the F gene revealed 100% identity with APV sequences described earlier.
dc.description79
dc.description237-41
dc.languageeng
dc.relationJournal Of Virological Methods
dc.relationJ. Virol. Methods
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBlotting, Southern
dc.subjectBrazil
dc.subjectChickens
dc.subjectDna, Viral
dc.subjectLuminescent Measurements
dc.subjectPneumovirus
dc.subjectPolymerase Chain Reaction
dc.subjectTurkeys
dc.subjectViral Fusion Proteins
dc.titleMolecular Characterization Of Brazilian Avian Pneumovirus Isolates: Comparison Between Immunochemiluminescent Southern Blot And Nested Pcr.
dc.typeArtículos de revistas


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