Insulin Receptor Has Tyrosine Kinase Activity Toward Shc In Rat Liver.

dc.creatorPáez-Espinosa, E V
dc.creatorCarvalho, C R
dc.creatorVelloso, L A
dc.creatorSaad, M J
dc.date1998-Nov
dc.date2015-11-27T12:19:28Z
dc.date2015-11-27T12:19:28Z
dc.date.accessioned2018-03-29T00:53:14Z
dc.date.available2018-03-29T00:53:14Z
dc.identifierBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 31, n. 11, p. 1415-9, 1998-Nov.
dc.identifier0100-879X
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/9921277
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194291
dc.identifier9921277
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294524
dc.descriptionInsulin induces tyrosine phosphorylation of Shc in cell cultures and in insulin-sensitive tissues of the intact rat. However, the ability of insulin receptor (IR) tyrosine kinase to phosphorylate Shc has not been previously demonstrated. In the present study, we investigated insulin-induced IR tyrosine kinase activity towards Shc. Insulin receptor was immunoprecipitated from liver extracts, before and after a very low dose of insulin into the portal vein, and incubated with immunopurified Shc from liver of untreated rats. The kinase assay was performed in vitro in the presence of exogenous ATP and the phosphorylation level was quantified by immunoblotting with antiphosphotyrosine antibody. The results demonstrate that Shc interacted with insulin receptor after infusion of insulin, and, more important, there was insulin receptor kinase activity towards immunopurified Shc. The description of this pathway in animal tissue may have an important role in insulin receptor tyrosine kinase activity toward mitogenic transduction pathways.
dc.description31
dc.description1415-9
dc.languageeng
dc.relationBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]
dc.relationBraz. J. Med. Biol. Res.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAdaptor Proteins, Signal Transducing
dc.subjectAdaptor Proteins, Vesicular Transport
dc.subjectAnimals
dc.subjectLiver
dc.subjectMale
dc.subjectPhosphorylation
dc.subjectPhosphotransferases
dc.subjectProtein-tyrosine Kinases
dc.subjectProteins
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReceptor, Insulin
dc.subjectShc Signaling Adaptor Proteins
dc.subjectSrc Homology Domains
dc.titleInsulin Receptor Has Tyrosine Kinase Activity Toward Shc In Rat Liver.
dc.typeArtículos de revistas


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