Cooperative Dna Binding And Sequence Discrimination By The Opaque2 Bzip Factor.

dc.creatorYunes, J A
dc.creatorVettore, A L
dc.creatorda Silva, M J
dc.creatorLeite, A
dc.creatorArruda, P
dc.date1998-Nov
dc.date2015-11-27T12:19:24Z
dc.date2015-11-27T12:19:24Z
dc.date.accessioned2018-03-29T00:53:07Z
dc.date.available2018-03-29T00:53:07Z
dc.identifierThe Plant Cell. v. 10, n. 11, p. 1941-55, 1998-Nov.
dc.identifier1040-4651
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/9811800
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194261
dc.identifier9811800
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294494
dc.descriptionThe maize Opaque2 (O2) protein is a basic leucine zipper transcription factor that controls the expression of distinct classes of endosperm genes through the recognition of different cis-acting elements in their promoters. The O2 target region in the promoter of the alpha-coixin gene was analyzed in detail and shown to comprise two closely adjacent binding sites, named O2u and O2d, which are related in sequence to the GCN4 binding site. Quantitative DNase footprint analysis indicated that O2 binding to alpha-coixin target sites is best described by a cooperative model. Transient expression assays showed that the two adjacent sites act synergistically. This synergy is mediated in part by cooperative DNA binding. In tobacco protoplasts, O2 binding at the O2u site is more important for enhancer activity than is binding at the O2d site, suggesting that the architecture of the O2-DNA complex is important for interaction with the transcriptional machinery.
dc.description10
dc.description1941-55
dc.languageeng
dc.relationThe Plant Cell
dc.relationPlant Cell
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectBase Sequence
dc.subjectBasic-leucine Zipper Transcription Factors
dc.subjectBinding Sites
dc.subjectChromosome Mapping
dc.subjectDna Probes
dc.subjectDna, Plant
dc.subjectDna-binding Proteins
dc.subjectEvolution, Molecular
dc.subjectG-box Binding Factors
dc.subjectGene Expression
dc.subjectGenes, Plant
dc.subjectLeucine Zippers
dc.subjectMolecular Sequence Data
dc.subjectMutagenesis, Site-directed
dc.subjectPlant Proteins
dc.subjectPromoter Regions, Genetic
dc.subjectTranscription Factors
dc.subjectZea Mays
dc.titleCooperative Dna Binding And Sequence Discrimination By The Opaque2 Bzip Factor.
dc.typeArtículos de revistas


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