Ca2+-stimulated Mitochondrial Reactive Oxygen Species Generation And Permeability Transition Are Inhibited By Dibucaine Or Mg2+.

dc.creatorKowaltowski, A J
dc.creatorNaia-da-Silva, E S
dc.creatorCastilho, R F
dc.creatorVercesi, A E
dc.date1998-Nov
dc.date2015-11-27T12:19:23Z
dc.date2015-11-27T12:19:23Z
dc.date.accessioned2018-03-29T00:53:05Z
dc.date.available2018-03-29T00:53:05Z
dc.identifierArchives Of Biochemistry And Biophysics. v. 359, n. 1, p. 77-81, 1998-Nov.
dc.identifier0003-9861
dc.identifier10.1006/abbi.1998.0870
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/9799563
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194253
dc.identifier9799563
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294486
dc.descriptionMitochondrial swelling and membrane protein thiol oxidation associated with mitochondrial permeability transition induced by Ca2+ and t-butyl hydroperoxide or inorganic phosphate, but not 4, 4'-diisothiocyanatostilbene-2,2'-disulfonic acid or phenylarsine oxide, are inhibited by the local anesthetic dibucaine. Dibucaine promotes an inhibition of the Ca2+-induced increase in mitochondrial H2O2 generation measured by the oxidation of scopoletin in the presence of horseradish peroxidase. This decrease in mitochondrial H2O2 generation may be attributed to the reduction of Ca2+ binding to the membrane induced by dibucaine, as assessed by measuring 45Ca2+ binding to the mitochondrial membrane. Mg2+ also inhibited Ca2+ binding to the mitochondrial membrane, mitochondrial swelling, membrane protein thiol oxidation, and H2O2 generation induced by Ca2+. Together, these results demonstrate that the mechanism by which dibucaine and Mg2+ inhibit mitochondrial permeability transition is related to the decrease in reactive oxygen species generation induced by Ca2+-promoted alterations of inner mitochondrial membrane properties.
dc.description359
dc.description77-81
dc.languageeng
dc.relationArchives Of Biochemistry And Biophysics
dc.relationArch. Biochem. Biophys.
dc.rightsfechado
dc.rightsCopyright 1998 Academic Press.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBinding Sites
dc.subjectBinding, Competitive
dc.subjectCalcium
dc.subjectCell Membrane Permeability
dc.subjectDibucaine
dc.subjectIntracellular Membranes
dc.subjectMagnesium
dc.subjectMitochondria, Liver
dc.subjectProtein Binding
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReactive Oxygen Species
dc.titleCa2+-stimulated Mitochondrial Reactive Oxygen Species Generation And Permeability Transition Are Inhibited By Dibucaine Or Mg2+.
dc.typeArtículos de revistas


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