| dc.creator | de Faria, L C | |
| dc.creator | Pasquini, C | |
| dc.creator | de Oliveira Neto, G | |
| dc.date | 1991-Apr | |
| dc.date | 2015-11-27T12:19:21Z | |
| dc.date | 2015-11-27T12:19:21Z | |
| dc.date.accessioned | 2018-03-29T00:53:01Z | |
| dc.date.available | 2018-03-29T00:53:01Z | |
| dc.identifier | The Analyst. v. 116, n. 4, p. 357-60, 1991-Apr. | |
| dc.identifier | 0003-2654 | |
| dc.identifier | | |
| dc.identifier | http://www.ncbi.nlm.nih.gov/pubmed/1872483 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/194235 | |
| dc.identifier | 1872483 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1294468 | |
| dc.description | A flow injection method was developed, aimed at the determination of urea in human serum. The system makes use of the naturally immobilized urease present in Canavalia ensiformis DC (jack bean). A column is filled with small pieces of this bean, and the sample (50 microliters) containing urea passes through it carried by a 1% NaCl solution. On leaving the column the stream is merged with an alkaline reagent (0.5 mol dm-3 NaOH; 0.5% disodium dihydrogen ethylenediaminetetraacetate). The ammonium ions, arising from the enzymatic reaction that occurs inside the column, are changed into the molecular form, which permeates a polytetrafluoroethylene membrane and is received in a de-ionized water acceptor stream. The ammonia ionizes causing an increase in the conductance, which is proportional to the urea content of the sample. About 40 samples can be processed in 1 h with negligible carry-over and with a relative standard deviation of 1% or less. The results are in agreement with those obtained by a standard spectrophotometric method. | |
| dc.description | 116 | |
| dc.description | 357-60 | |
| dc.language | eng | |
| dc.relation | The Analyst | |
| dc.relation | Analyst | |
| dc.rights | aberto | |
| dc.rights | | |
| dc.source | PubMed | |
| dc.subject | Conductometry | |
| dc.subject | Enzymes, Immobilized | |
| dc.subject | Humans | |
| dc.subject | Hydrogen-ion Concentration | |
| dc.subject | Urea | |
| dc.subject | Urease | |
| dc.title | Determination Of Urea In Serum By Using Naturally Immobilized Urease In A Flow Injection Conductimetric System. | |
| dc.type | Artículos de revistas | |
