Large-scale Production And Purification Of Recombinant Protein From An Insect Cell/baculovirus System In Erlenmeyer Flasks: Application To The Chicken Poly(adp-ribose) Polymerase Catalytic Domain.

dc.creatorMiranda, E A
dc.creatorde-Murcia, G
dc.creatorMénissier-de-Murcia, J
dc.date1997-Aug
dc.date2015-11-27T12:19:01Z
dc.date2015-11-27T12:19:01Z
dc.date.accessioned2018-03-29T00:52:22Z
dc.date.available2018-03-29T00:52:22Z
dc.identifierBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 30, n. 8, p. 923-8, 1997-Aug.
dc.identifier0100-879X
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/9361719
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194065
dc.identifier9361719
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294298
dc.descriptionA simple and inexpensive shaker/Erlenmeyer flask system for large-scale cultivation of insect cells is described and compared to a commercial spinner system. On the basis of maximum cell density, average population doubling time and overproduction of recombinant protein, a better result was obtained with a simpler and less expensive bioreactor consisting of Erlenmeyer flasks and an ordinary shaker waterbath. Routinely, about 90 mg of pure poly(ADP-ribose) polymerase catalytic domain was obtained for a total of 3 x 10(9) infected cells in three liters of culture.
dc.description30
dc.description923-8
dc.languageeng
dc.relationBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]
dc.relationBraz. J. Med. Biol. Res.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBaculoviridae
dc.subjectIn Vitro Techniques
dc.subjectInsects
dc.subjectPoly Adenosine Diphosphate Ribose
dc.subjectPolynucleotide Adenylyltransferase
dc.subjectRecombinant Proteins
dc.titleLarge-scale Production And Purification Of Recombinant Protein From An Insect Cell/baculovirus System In Erlenmeyer Flasks: Application To The Chicken Poly(adp-ribose) Polymerase Catalytic Domain.
dc.typeArtículos de revistas


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