Opening Of The Mitochondrial Permeability Transition Pore By Uncoupling Or Inorganic Phosphate In The Presence Of Ca2+ Is Dependent On Mitochondrial-generated Reactive Oxygen Species.

dc.creatorKowaltowski, A J
dc.creatorCastilho, R F
dc.creatorVercesi, A E
dc.date1996-Jan
dc.date2015-11-27T12:18:42Z
dc.date2015-11-27T12:18:42Z
dc.date.accessioned2018-03-29T00:51:43Z
dc.date.available2018-03-29T00:51:43Z
dc.identifierFebs Letters. v. 378, n. 2, p. 150-2, 1996-Jan.
dc.identifier0014-5793
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/8549822
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/193898
dc.identifier8549822
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294131
dc.descriptionIn this study, we show that mitochondrial membrane permeability transition in Ca(2+)-loaded mitochondria treated with carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP) or inorganic phosphate (P(i)) is preceded by enhanced production of H2O2. This production is inhibited either by ethylene glycobis(b-aminoethyl ether)N,N,N',N'-tetraacetic acid (EGTA) or Mg2+, but not by cyclosporin A. Permeability transition is prevented either by EGTA, catalase or dithiothreitol, suggesting the involvement of Ca2+, H2O2 and oxidation of membrane protein thiols in this mechanism. When mitochondria are incubated under anaerobiosis, no permeabilization or H2O2 production occurs. Based on these results we conclude that mitochondrial permeability transition induced by P(i) or FCCP-uncoupling is dependent on mitochondrial-generated reactive oxygen species.
dc.description378
dc.description150-2
dc.languageeng
dc.relationFebs Letters
dc.relationFEBS Lett.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCalcium
dc.subjectCarbonyl Cyanide P-trifluoromethoxyphenylhydrazone
dc.subjectCatalase
dc.subjectDithiothreitol
dc.subjectEgtazic Acid
dc.subjectHydrogen Peroxide
dc.subjectIntracellular Membranes
dc.subjectMagnesium
dc.subjectMitochondria, Liver
dc.subjectPermeability
dc.subjectPhosphates
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReactive Oxygen Species
dc.subjectUncoupling Agents
dc.titleOpening Of The Mitochondrial Permeability Transition Pore By Uncoupling Or Inorganic Phosphate In The Presence Of Ca2+ Is Dependent On Mitochondrial-generated Reactive Oxygen Species.
dc.typeArtículos de revistas


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