Optical Anisotropy Of Toluidine Blue-stained Transformed Cell Nuclei.

dc.creatorMello, M L
dc.date1995-Apr
dc.date2015-11-27T12:18:35Z
dc.date2015-11-27T12:18:35Z
dc.date.accessioned2018-03-29T00:51:28Z
dc.date.available2018-03-29T00:51:28Z
dc.identifierActa Histochemica. v. 97, n. 2, p. 159-64, 1995-Apr.
dc.identifier0065-1281
dc.identifier10.1016/S0065-1281(11)80093-0
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/7660733
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/193829
dc.identifier7660733
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294062
dc.descriptionChanges in chromatin order with cell transformation were studied in terms of optical anisotropy (birefringence) in toluidine blue-stained NIH 3T3 and Balb/3T3 cells. The transformed NIH 3T3 cell lines used were obtained by transfection with the T24 cell H-ras oncogene and the whole genomic DNA of MCF-7 human breast carcinoma cells. C2PO and PAP2 cell lines were used as representatives of Ha-ras-transformed NIH 3T3 cells with poor and high metastatic ability, respectively. The chromatin of all cells examined exhibited metachromasy and a birefringence of greenish-yellow interference color. The highest birefringence intensity was found in the chromatin granules of the most frequent nuclear phenotype of ras- and MCF-7 DNA-transformed cells, with the exception of the cells with high metastatic ability, in which a faint birefringence was observed. The differences in chromatin birefringence intensity are assumed to indicate differences in chromatin stereoarrangement with cell transformation. In the case of the highly tumorigenic and/or highly metastatic transformants the faint birefringence is assumed to be associated with the heterogeneous and complex physiological processes that may require a relatively less ordered arrangement of the chromatin.
dc.description97
dc.description159-64
dc.languageeng
dc.relationActa Histochemica
dc.relationActa Histochem.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subject3t3 Cells
dc.subjectAnimals
dc.subjectBirefringence
dc.subjectBreast Neoplasms
dc.subjectCell Nucleus
dc.subjectCell Transformation, Neoplastic
dc.subjectDna, Neoplasm
dc.subjectGenes, Ras
dc.subjectHumans
dc.subjectMice
dc.subjectMice, Inbred Balb C
dc.subjectMice, Inbred Strains
dc.subjectTolonium Chloride
dc.subjectTransfection
dc.subjectTumor Cells, Cultured
dc.titleOptical Anisotropy Of Toluidine Blue-stained Transformed Cell Nuclei.
dc.typeArtículos de revistas


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