Chemical And Photochemical Generated Carbon-centered Radical Intermediate And Its Reaction With Desoxyribonucleic Acid.

dc.creatorJusto, G Z
dc.creatorLivotto, P R
dc.creatorDurán, N
dc.date1995-Oct
dc.date2015-11-27T12:18:34Z
dc.date2015-11-27T12:18:34Z
dc.date.accessioned2018-03-29T00:51:25Z
dc.date.available2018-03-29T00:51:25Z
dc.identifierFree Radical Biology & Medicine. v. 19, n. 4, p. 431-40, 1995-Oct.
dc.identifier0891-5849
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/7590392
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/193819
dc.identifier7590392
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294052
dc.descriptionThe possible action of carbon-centered radicals in promoting damage to DNA is explored by generation of the 2-phenylethyl radical. The radical is generated during oxidation of phenelzine (2-phenylethyl hydrazine) by ferricyanide, as well as optically from phenylpropionic acid. Covalent binding of the 2-phenylethyl radical to DNA is suggested by studies with the plasmid pBR 322 DNA. Other sensitive techniques used to study DNA damage were the interaction with formaldehyde at 60 degrees C and the fluorescence of DNA-Tb(III) and DNA-DAPI complexes. Theoretical MNDO calculations indicated a preferential attack at position 8 of the guanine residues. This study shows that the 2-phenylethyl radical is able to induce primary effects on nucleic acid structure, leading to alkylated products, especially at purine rings.
dc.description19
dc.description431-40
dc.languageeng
dc.relationFree Radical Biology & Medicine
dc.relationFree Radic. Biol. Med.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAlkylation
dc.subjectDna
dc.subjectDna Damage
dc.subjectElectron Spin Resonance Spectroscopy
dc.subjectFerricyanides
dc.subjectFluorescent Dyes
dc.subjectFree Radicals
dc.subjectIndoles
dc.subjectIntercalating Agents
dc.subjectOxidation-reduction
dc.subjectPhenelzine
dc.subjectPhenylpropionates
dc.subjectPhotolysis
dc.subjectSpectrometry, Fluorescence
dc.subjectTerbium
dc.titleChemical And Photochemical Generated Carbon-centered Radical Intermediate And Its Reaction With Desoxyribonucleic Acid.
dc.typeArtículos de revistas


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