Effects Of Glucose On 45ca2+ Outflow, Cytosolic Ca2+ Concentration And Insulin Release From Freshly Isolated And Cultured Adult Rat Islets.

dc.creatorBoschero, A C
dc.creatorBordin, S
dc.creatorHerchuelz, A
dc.creatorLebrun, P
dc.date1990-Oct
dc.date2015-11-27T12:18:30Z
dc.date2015-11-27T12:18:30Z
dc.date.accessioned2018-03-29T00:51:17Z
dc.date.available2018-03-29T00:51:17Z
dc.identifierCell Calcium. v. 11, n. 9, p. 603-9, 1990-Oct.
dc.identifier0143-4160
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/2285929
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/193784
dc.identifier2285929
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294017
dc.descriptionThe present study aimed at comparing the effects of glucose on ionic and secretory events in freshly isolated and 5-7 day cultured rat pancreatic islets. The capacity of glucose to provoke insulin release was severely reduced in islets maintained in culture. Whether in freshly isolated or cultured islets, glucose provoked a marked and sustained decrease in 45Ca2+ outflow from islets deprived of extracellular Ca2+. In the presence of extracellular Ca2+ throughout, the magnitude of the glucose-induced secondary rise in 45Ca2+ outflow was reduced in cultured islets. Glucose provoked a weaker increase in [Ca2+]i in islet cells obtained from cultured islets than in islet cells dissociated from freshly isolated pancreatic islets. On the other hand, the stimulatory effect of carbamylcholine on 45Ca2+ outflow was unaffected by tissue culture. Lastly, in islet cells obtained from cultured islets, the increase in [Ca2+]i evoked by K+ depolarization averaged half of that observed in control experiments. These results indicate that the reduced secretory potential of glucose in cultured pancreatic islets can be ascribed to the inability of the nutrient secretagogue to provoke a suitable increase in Ca2+ influx.
dc.description11
dc.description603-9
dc.languageeng
dc.relationCell Calcium
dc.relationCell Calcium
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCalcium
dc.subjectCarbachol
dc.subjectCell Separation
dc.subjectCells, Cultured
dc.subjectCytosol
dc.subjectFemale
dc.subjectGlucose
dc.subjectInsulin
dc.subjectIslets Of Langerhans
dc.subjectKinetics
dc.subjectPotassium Chloride
dc.subjectRats
dc.subjectRats, Inbred Strains
dc.titleEffects Of Glucose On 45ca2+ Outflow, Cytosolic Ca2+ Concentration And Insulin Release From Freshly Isolated And Cultured Adult Rat Islets.
dc.typeArtículos de revistas


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