Structural Characterization And Promoter Activity Analysis Of The Gamma-kafirin Gene From Sorghum.

dc.creatorde Freitas, F A
dc.creatorYunes, J A
dc.creatorda Silva, M J
dc.creatorArruda, P
dc.creatorLeite, A
dc.date1994-Oct
dc.date2015-11-27T12:18:27Z
dc.date2015-11-27T12:18:27Z
dc.date.accessioned2018-03-29T00:51:12Z
dc.date.available2018-03-29T00:51:12Z
dc.identifierMolecular & General Genetics : Mgg. v. 245, n. 2, p. 177-86, 1994-Oct.
dc.identifier0026-8925
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/7816025
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/193763
dc.identifier7816025
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1293996
dc.descriptionA genomic clone encoding the gamma-kafirin gene from sorghum was isolated and sequenced. A 2938 bp sequenced fragment includes an intronless open reading frame of 636 nucleotides encoding a putative polypeptide of 212 amino acids. Comparison of the deduced amino acid sequence of gamma-kafirin with the published sequences of gamma-prolamins of maize, and Coix revealed highly conserved domains. The N-terminal region of these proteins contains the conserved hexapeptide PPPVHL, which is repeated eight times in gamma-zein, four times in gamma-kafirin and three times in gamma-coixin. The number of PPPVHL repeats accounts predominantly for the differences in the molecular weights of gamma-prolamins. Several putative regulatory sequences common to the gamma-kafirin and gamma-zein genes were identified in both the 5' and the 3' flanking regions. Putative GCN4-like regulatory sequences were found at positions -192 and -476 in the 5' flanking region of gamma-kafirin. In the 3' noncoding region, three putative polyadenylation signals, two AATAAT and one AATGAA, were found at positions +658, +716, and +785, respectively. In order to investigate the role of the putative GCN4-like motifs and other possible cis-acting element(s) of the gamma-kafirin promoter, a series of deleted and chimeric promoter constructs were introduced into maize, Coix and sorghum tissues by particle bombardment. Histochemical analysis of beta-glucuronidase (GUS) activity in different tissues indicated that the element(s) responsible for tissue specificity is probably located in the 285-bp proximal region of the promoter, while the remaining promoter sequence seems to carry the element(s) responsible for the quantitative response.
dc.description245
dc.description177-86
dc.languageeng
dc.relationMolecular & General Genetics : Mgg
dc.relationMol. Gen. Genet.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectCereals
dc.subjectGenes, Plant
dc.subjectMolecular Sequence Data
dc.subjectPlant Proteins
dc.subjectPromoter Regions, Genetic
dc.subjectSeeds
dc.subjectSequence Alignment
dc.subjectSequence Homology, Amino Acid
dc.titleStructural Characterization And Promoter Activity Analysis Of The Gamma-kafirin Gene From Sorghum.
dc.typeArtículos de revistas


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