Immunological Response To Cell-free Antigens Of Paracoccidioides Brasiliensis: Relationship With Clinical Forms Of Paracoccidioidomycosis.

dc.creatorBlotta, M H
dc.creatorCamargo, Z P
dc.date1993-Mar
dc.date2015-11-27T12:18:25Z
dc.date2015-11-27T12:18:25Z
dc.date.accessioned2018-03-29T00:51:07Z
dc.date.available2018-03-29T00:51:07Z
dc.identifierJournal Of Clinical Microbiology. v. 31, n. 3, p. 671-6, 1993-Mar.
dc.identifier0095-1137
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/8458961
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/193739
dc.identifier8458961
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1293972
dc.descriptionSera from patients with the acute (AF) and chronic (CF) forms of paracoccidioidomycosis (PCM) were tested against Paracoccidioides brasiliensis cell-free antigens by Western blot (immunoblot). The CFA preparation contained components ranging in molecular mass from 18 to 102 kDa. The immunoglobulin G (IgG) reactivity profiles were similar for patients with both forms of the disease, and the 43-kDa component was recognized by 100% of the sera. IgM antibodies from the AF- and the CF-PCM sera recognized 21 and 20 components, respectively, the AF-PCM sera reacting preferentially with components with molecular masses above 50 kDa. None of the AF-PCM sera (IgM) reacted with the 43-kDa component, and only 10% of the CF-PCM sera recognized this molecule. The IgA response was more significant in the CF-PCM group than in the AF-PCM group, and the 43- and 74-kDa components were the most reactive ones (about 40% each). Our results showed that the cell-free antigen preparation is very appropriate for the immunoblotting analysis of PCM sera, and they also showed that the detection of IgG anti-gp43 is the best marker for the diagnosis and the following up of patients with the acute or the chronic form of the disease.
dc.description31
dc.description671-6
dc.languageeng
dc.relationJournal Of Clinical Microbiology
dc.relationJ. Clin. Microbiol.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAcute Disease
dc.subjectAdolescent
dc.subjectAdult
dc.subjectAged
dc.subjectAntibodies, Fungal
dc.subjectAntigens, Fungal
dc.subjectBlotting, Western
dc.subjectChild
dc.subjectChronic Disease
dc.subjectHumans
dc.subjectImmunoglobulin A
dc.subjectImmunoglobulin G
dc.subjectImmunoglobulin M
dc.subjectMiddle Aged
dc.subjectParacoccidioidomycosis
dc.titleImmunological Response To Cell-free Antigens Of Paracoccidioides Brasiliensis: Relationship With Clinical Forms Of Paracoccidioidomycosis.
dc.typeArtículos de revistas


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