dc.creatorCondino-Neto, A
dc.creatorMuscará, M N
dc.creatorGrumach, A S
dc.creatorCarneiro-Sampaio, M M
dc.creatorDe Nucci, G
dc.date1993-May
dc.date2015-11-27T12:18:24Z
dc.date2015-11-27T12:18:24Z
dc.date.accessioned2018-03-29T00:51:06Z
dc.date.available2018-03-29T00:51:06Z
dc.identifierBritish Journal Of Clinical Pharmacology. v. 35, n. 5, p. 485-90, 1993-May.
dc.identifier0306-5251
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/8390277
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/193734
dc.identifier8390277
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1293967
dc.description1. Chronic granulomatous disease (CGD) is a group of genetic disorders characterised by recurrent severe suppurative infections due to impaired microbial killing. The principal biochemical defect is an impairment in the production of reactive oxygen intermediates by phagocytes. 2. Nitric oxide (NO) is synthesised from the guanidino nitrogen atom(s) of L-arginine and has recently been proposed to be involved in defence mechanisms. The aim of this study was to investigate the involvement of the oxidative burst in the biosynthesis of NO by neutrophils and mononuclear cells from patients with CGD. 3. NO synthesis was assayed by the ability of neutrophils and mononuclear cells to inhibit thrombin-induced washed platelet aggregation while superoxide anion (O2-) production was measured spectrophotometrically by the superoxide dismutase inhibitable reduction of cytochrome c. 4. Neutrophils and mononuclear cells from patients with CGD released NO. This release was inhibited by nitro-L-arginine methyl ester but could be reversed by L-arginine. Zymosan- and PMA-induced O2- production was less than 10% as compared with healthy controls. 5. These results indicate that O2- production is not essential for NO synthesis in human leucocytes.
dc.description35
dc.description485-90
dc.languageeng
dc.relationBritish Journal Of Clinical Pharmacology
dc.relationBr J Clin Pharmacol
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectArginine
dc.subjectBlood Platelets
dc.subjectChild
dc.subjectFemale
dc.subjectGranulomatous Disease, Chronic
dc.subjectHumans
dc.subjectIn Vitro Techniques
dc.subjectInfant
dc.subjectMale
dc.subjectMonocytes
dc.subjectNg-nitroarginine Methyl Ester
dc.subjectNeutrophils
dc.subjectNitric Oxide
dc.subjectPlatelet Aggregation
dc.subjectRespiratory Burst
dc.subjectSuperoxides
dc.subjectThrombin
dc.titleNeutrophils And Mononuclear Cells From Patients With Chronic Granulomatous Disease Release Nitric Oxide.
dc.typeArtículos de revistas


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