The Plant Mitochondrial Open Reading Frame Orf221 Encodes A Membrane-bound Protein.

dc.creatorPrioli, L M
dc.creatorHuang, J
dc.creatorLevings, C S
dc.date1993-Oct
dc.date2015-11-27T12:18:19Z
dc.date2015-11-27T12:18:19Z
dc.date.accessioned2018-03-29T00:50:57Z
dc.date.available2018-03-29T00:50:57Z
dc.identifierPlant Molecular Biology. v. 23, n. 2, p. 287-95, 1993-Oct.
dc.identifier0167-4412
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/8219065
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/193699
dc.identifier8219065
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1293932
dc.descriptionWe have shown that the open reading frame orf221 is an active mitochondrial gene which encodes a novel mitochondrial polypeptide. The orf221 sequence is common to higher plants but absent in animal and fungal mitochondria. A mitochondrial polypeptide with an apparent molecular weight of 21,000 was detected with a polyclonal antibody raised against an ORF221 fusion protein. In organello translation followed by immunoprecipitation with the anti-ORF221 antibody demonstrated that this polypeptide is encoded by the orf221 gene in plant mitochondria. The ORF221 was found to be a mitochondrial membrane protein in normal (N), cms-T, and cms-C cytoplasms of several inbred lines of maize (Zea mays L.) and in other plant species.
dc.description23
dc.description287-95
dc.languageeng
dc.relationPlant Molecular Biology
dc.relationPlant Mol. Biol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectCell Fractionation
dc.subjectDna, Mitochondrial
dc.subjectEscherichia Coli
dc.subjectGenes, Plant
dc.subjectMembrane Proteins
dc.subjectOpen Reading Frames
dc.subjectPlants
dc.subjectProtein Biosynthesis
dc.subjectRecombinant Fusion Proteins
dc.subjectZea Mays
dc.titleThe Plant Mitochondrial Open Reading Frame Orf221 Encodes A Membrane-bound Protein.
dc.typeArtículos de revistas


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