Lipoxygenase-derived Mediators May Be Involved In In Vivo Neutrophil Migration Induced By Bothrops Erythromelas And Bothrops Alternatus Venoms.

dc.creatorFlores, C A
dc.creatorZappellini, A
dc.creatorPrado-Franceschi, J
dc.date1993-Dec
dc.date2015-11-27T12:18:18Z
dc.date2015-11-27T12:18:18Z
dc.date.accessioned2018-03-29T00:50:55Z
dc.date.available2018-03-29T00:50:55Z
dc.identifierToxicon : Official Journal Of The International Society On Toxinology. v. 31, n. 12, p. 1551-9, 1993-Dec.
dc.identifier0041-0101
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/8146868
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/193691
dc.identifier8146868
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1293924
dc.descriptionBothrops erythromelas (BEV) and B. alternatus (BAV) venoms induced a dose-dependent neutrophil migration when injected into rat peritoneal cavities (20-160 micrograms/cavity). These venoms (80 micrograms/rat) also induced neutrophil migration in the air pouch model of inflammation. This migratory response seemed to be related to the phospholipase A2 (PLA2) activity of the venoms. BAV had approximately two times more PLA2 activity than BEV, and the neutrophil migration induced by the former venom was two to three-fold greater than that observed with the latter. Heated (90 degrees C for 5 min) BEV lost about 50% of its PLA2 activity and this was accompanied by a corresponding loss in the ability to induce neutrophil chemotaxis. Dexamethasone (0.5 mg/kg, s.c.), an indirect inhibitor of PLA2 activity, also abolished the neutrophil migration induced by both venoms. Since NDGA (100 mg/kg, s.c.) and dexamethasone, but not indomethacin (2 mg/kg, s.c.), strongly reduced the neutrophil migration induced by both bothropic venoms, it is suggested that arachidonate-derived lipoxygenase metabolites such as leukotriene B4 act as the chemotactic mediators. Macrophages could be the main cellular source of such metabolites since they are the predominant resident cells in the rat air pouch, and the migratory response of BEV and BAV into peritoneal cavities was potentiated in rats pretreated with thioglycollate. The neutrophil migration induced by BEV and BAV was not due to endotoxin contamination since heated BEV showed no effect and polymyxin B-treated BAV still remained active.
dc.description31
dc.description1551-9
dc.languageeng
dc.relationToxicon : Official Journal Of The International Society On Toxinology
dc.relationToxicon
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectArachidonic Acids
dc.subjectBothrops
dc.subjectChemotactic Factors
dc.subjectChemotaxis, Leukocyte
dc.subjectCrotalid Venoms
dc.subjectDexamethasone
dc.subjectFemale
dc.subjectIndomethacin
dc.subjectLipoxygenase
dc.subjectMacrophages, Peritoneal
dc.subjectMale
dc.subjectMasoprocol
dc.subjectNeutrophils
dc.subjectPhospholipases A
dc.subjectPhospholipases A2
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectThioglycolates
dc.titleLipoxygenase-derived Mediators May Be Involved In In Vivo Neutrophil Migration Induced By Bothrops Erythromelas And Bothrops Alternatus Venoms.
dc.typeArtículos de revistas


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