Spermine Stimulates Rna-dependent Reverse Transcriptase Activity.

dc.creatorAoyama, H
dc.date1989-Jul
dc.date2015-11-27T12:10:04Z
dc.date2015-11-27T12:10:04Z
dc.date.accessioned2018-03-29T00:50:35Z
dc.date.available2018-03-29T00:50:35Z
dc.identifierBiochemistry International. v. 19, n. 1, p. 67-76, 1989-Jul.
dc.identifier0158-5231
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/2476130
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/193607
dc.identifier2476130
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1293840
dc.descriptionThe polyamine spermine can not replace Mg2+ in the reactions catalyzed by avian myeloblastosis virus reverse transcriptase. In the presence of suboptimal Mg2+ concentrations, spermine enhanced the reverse transcriptase activities with viral 70 S RNA and synthetic polyriboadenylic acid as templates, about 4-fold and 2-fold, respectively. A similar effect occurred in the polyriboadenylic acid-directed reaction using Mn2+ as divalent cation. On the other hand, no stimulation by spermine was observed with activated DNA and polydeoxyadenylic acid as template in the reactions catalyzed by reverse transcriptase.
dc.description19
dc.description67-76
dc.languageeng
dc.relationBiochemistry International
dc.relationBiochem. Int.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAvian Leukosis Virus
dc.subjectAvian Myeloblastosis Virus
dc.subjectCations, Divalent
dc.subjectDna
dc.subjectMagnesium
dc.subjectPoly Da-dt
dc.subjectRna, Viral
dc.subjectRna-directed Dna Polymerase
dc.subjectSpermine
dc.subjectTemplates, Genetic
dc.titleSpermine Stimulates Rna-dependent Reverse Transcriptase Activity.
dc.typeArtículos de revistas


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