Artículos de revistas
Ca2+-dependent Nad(p)+-induced Alterations Of Rat Liver And Hepatoma Mitochondrial Membrane Permeability.
Registro en:
Biochemical And Biophysical Research Communications. v. 154, n. 3, p. 934-41, 1988-Aug.
0006-291X
3136771
Autor
Vercesi, A E
Ferraz, V L
Macedo, D V
Fiskum, G
Institución
Resumen
Deenergized rat liver or AS-30D hepatoma mitochondria underwent extensive swelling when incubated in the presence of Ca2+ and an oxidant of mitochondrial pyridine nucleotides. This swelling was stimulated by phosphate and inhibited by ruthenium red, Mg2+ plus ATP and dibucaine. The degree of inhibition by dibucaine was shown to vary inversely with the Ca2+ concentration. The inhibition caused by ruthenium red was completely removed by the Ca2+ ionophore ionomycin indicating that the effect of the cation is mediated by internal binding sites. Since mitochondria were totally deenergized the data definitely rule out the requirement for Ca2+ cycling across the membrane in this mechanism. 154 934-41