DNA-based typing of blood groups for the management of multiply-transfused sickle cell disease patients

dc.creatorCastilho, L
dc.creatorRios, M
dc.creatorBianco, C
dc.creatorPellegerino, J
dc.creatorAlberto, FL
dc.creatorSaad, STO
dc.creatorCosta, FF
dc.date2002
dc.dateFEB
dc.date2014-11-13T16:41:24Z
dc.date2015-11-26T18:08:05Z
dc.date2014-11-13T16:41:24Z
dc.date2015-11-26T18:08:05Z
dc.date.accessioned2018-03-29T00:50:11Z
dc.date.available2018-03-29T00:50:11Z
dc.identifierTransfusion. Amer Assoc Blood Banks, v. 42, n. 2, n. 232, n. 238, 2002.
dc.identifier0041-1132
dc.identifierWOS:000174446900017
dc.identifier10.1046/j.1537-2995.2002.00029.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/62410
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/62410
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/62410
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1293740
dc.descriptionBACKGROUND: The usefulness of DNA genotyping for RBC antigens as a tool for the management of multiply-transfused patients with sickle cell disease (SCD) to overcome the limitations of hernagglutination assays was evaluated. STUDY DESIGN AND METHODS: Blood samples from 40 multiply-transfused SCD patients were studied by hemagglutination and by PCR-RFLP for antigens or genes in the Rh (D, C/c, E/e), Kell, Kidd, and Duffy systems. RESULTS: Discrepancies were found between hemagglutination and DNA typing test results in six patients: two were discrepant in Rh typing (one was D- by hemagglutination and RhD by DNA, and one was E+e- and RhEe by DNA), two were discrepant in Duffy typing [both were Fy(a+b-) and Fy(b)/Fy(b) by DNA], and four were discrepant in Kidd typing [Jk(a+b+) and Jk(b)/Jk(b) by DNA; two of these samples were also discrepant in Duffy]. Stored segments from blood units that had been recently transfused to these six recipients were phenotyped, confirming that the transfused RBCs were the source of the discrepancy between genotype and phenotype. CONCLUSION: DNA typing of blood groups by PCR-RFLP in peripheral blood WBCs contributes to the management of transfusions in SCD patients by allowing a more accurate selection of donor units.
dc.description42
dc.description2
dc.description232
dc.description238
dc.languageen
dc.publisherAmer Assoc Blood Banks
dc.publisherBethesda
dc.publisherEUA
dc.relationTransfusion
dc.relationTransfusion
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectRhd Gene
dc.subjectAlloimmunization
dc.subjectExpression
dc.subjectRhce
dc.subjectPhenotype
dc.subjectAfricans
dc.subjectSamples
dc.subjectBlacks
dc.titleDNA-based typing of blood groups for the management of multiply-transfused sickle cell disease patients
dc.typeArtículos de revistas


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