A low molecular weight enterotoxic hemolysin from clinical Enterobacter cloacae

dc.creatorSimi, S
dc.creatorCarbonell, GV
dc.creatorFalcon, RM
dc.creatorGatti, MSV
dc.creatorJoazeiro, PP
dc.creatorDarini, AL
dc.creatorYano, T
dc.date2003
dc.dateJUL
dc.date2014-11-13T14:23:48Z
dc.date2015-11-26T18:07:48Z
dc.date2014-11-13T14:23:48Z
dc.date2015-11-26T18:07:48Z
dc.date.accessioned2018-03-29T00:49:56Z
dc.date.available2018-03-29T00:49:56Z
dc.identifierCanadian Journal Of Microbiology. Natl Research Council Canada, v. 49, n. 7, n. 479, n. 482, 2003.
dc.identifier0008-4166
dc.identifierWOS:000186118600009
dc.identifier10.1139/W03-060
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/76019
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/76019
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/76019
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1293675
dc.descriptionSeven of 50 Enterobacter cloacae strains from clinical isolates produced small turbid zones of hemolysis in horse and sheep blood agar plates, and the culture supernatants were also positive for hemolytic activity. The hemolysin was partially purified from the culture supernatant of E. cloacae by ultrafiltration (PM-10 membrane) and extraction with acetone. Semipurified hemolysin was stable to heating (100degreesC, 30 min) and was soluble in organic solvents (acetone, ethanol, and methanol). The toxin showed no loss of biological activity after treatment with trypsin and was stable to acid treatment at pH 2.0 but not at a pH greater than 7.0. In the rat intestinal loop assay, the hemolysin caused hemorrhagic fluid accumulation and severe histological alterations. These findings indicate that this hemolysin may be a putative virulence factor in E. cloacae infections.
dc.description49
dc.description7
dc.description479
dc.description482
dc.languageen
dc.publisherNatl Research Council Canada
dc.publisherOttawa
dc.publisherCanadá
dc.relationCanadian Journal Of Microbiology
dc.relationCan. J. Microbiol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectEnterobacter cloacae
dc.subjecthemolysin
dc.subjectenterotoxin
dc.subjectHeat-stable Enterotoxin
dc.subjectEscherichia-coli
dc.subjectAeromonas-hydrophila
dc.subjectVibrio-cholerae
dc.subjectPurification
dc.subjectToxin
dc.subjectIntestine
dc.subjectBacteria
dc.titleA low molecular weight enterotoxic hemolysin from clinical Enterobacter cloacae
dc.typeArtículos de revistas


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