dc.creatorBertazzo, S
dc.creatorBertran, CA
dc.date2008
dc.dateJAN
dc.date2014-11-13T11:29:03Z
dc.date2015-11-26T18:07:11Z
dc.date2014-11-13T11:29:03Z
dc.date2015-11-26T18:07:11Z
dc.date.accessioned2018-03-29T00:49:18Z
dc.date.available2018-03-29T00:49:18Z
dc.identifierJournal Of Inorganic Biochemistry. Elsevier Science Inc, v. 102, n. 1, n. 137, n. 145, 2008.
dc.identifier0162-0134
dc.identifierWOS:000252523200014
dc.identifier10.1016/j.jinorgbio.2007.07.031
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64416
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/64416
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64416
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1293527
dc.descriptionOver the last 30 years several techniques have been developed to separate bone matrix and bone mineral, in order to allow for a study of each component independently of the other. Preservation of original characteristics of the phase studied after isolation has always been a great challenge for all such techniques. The hydrazine deproteination procedure, first proposed by Termine, has been one of the processes most widely used for studying bone mineral. It is found to be one of the most effective, notwithstanding controversy over its efficiency in bone deproteination and criticism regarding possible changes it could make to the characteristics of bone mineral. In this work, we have studied the possible chemical and physical alterations caused by the hydrazine deproteination process to bone mineral from rats and to other materials of biological interest. Materials were characterized by scanning electron microscopy (SEM), thermo-gravimetric analysis (TGA), X-ray diffractometry (XRD), inductive coupled plasma-optical emission spectroscopy (ICP-OES), C-H-N analysis and infrared spectroscopy (FTIR), before and after hydrazine deproteination. Finally, here we present a comprehensive discussion on the criticism of hydrazine deproteination. The experimental results obtained in this work, even when compared to the results in the literature, show that most widespread criticism to the hydrazine deproteination process is not completely justified. (c) 2007 Elsevier Inc. All rights reserved.
dc.description102
dc.description1
dc.description137
dc.description145
dc.languageen
dc.publisherElsevier Science Inc
dc.publisherNew York
dc.publisherEUA
dc.relationJournal Of Inorganic Biochemistry
dc.relationJ. Inorg. Biochem.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectdeproteination
dc.subjectbone
dc.subjectbone mineral
dc.subjecthydrazine
dc.subjectcalcium phosphate
dc.subjectX-ray-diffraction
dc.subjectState Nmr-spectroscopy
dc.subjectFemoral Cortical Bone
dc.subjectCalcium-phosphate
dc.subjectHydroxyapatite
dc.subjectCrystallinity
dc.subjectAge
dc.subjectIdentification
dc.subjectSolubility
dc.subjectScattering
dc.titleEffect of hydrazine deproteination on bone mineral phase: A critical view
dc.typeArtículos de revistas


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