Irradiated cationic mesoporphyrin rat liver mitochondria induces larger damage to isolated than the anionic form

dc.creatorInada, NM
dc.creatorda Silva, AR
dc.creatorJorge, RA
dc.creatorBorecky, J
dc.creatorVercesi, AE
dc.date2007
dc.date42005
dc.date2014-11-19T23:15:40Z
dc.date2015-11-26T18:07:06Z
dc.date2014-11-19T23:15:40Z
dc.date2015-11-26T18:07:06Z
dc.date.accessioned2018-03-29T00:49:14Z
dc.date.available2018-03-29T00:49:14Z
dc.identifierArchives Of Biochemistry And Biophysics. Elsevier Science Inc, v. 457, n. 2, n. 217, n. 224, 2007.
dc.identifier0003-9861
dc.identifierWOS:000243572800011
dc.identifier10.1016/j.abb.2006.10.025
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60734
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/60734
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60734
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1293509
dc.descriptionThe action of irradiated cationic Fe(III)TMPyP and anionic Fe(III)TPPS4 forms of mesoporphyrins on mitochondrial functions was investigated using experimental condition that caused minimal effects on mitochondria in the dark. Treatment of mitochondria with 1 mu M Fe(III)TMPyP for 2 min decreased the respiratory control by 3% in the dark and 28% after irradiation. Fe(III)TPPS4 (1 mu M) had no significant effect oil respiratory control under any of the above conditions. Both porphyrins increased the mitochondrial production of reactive oxygen species in the presence of Ca2+; however, the effect of Fe(III)TMPyP was significantly stronger. In both cases, this overproduction was associated with membrane lipid peroxidation. It was also observed that the association constant of Fe(III)TMPyP with mitochondria was I I times higher than that of Fe(III)TPPS4. In Conclusion, the damage to isolated mitochondria induced by Fe(III)TMPyP under illumination was larger than by Fe(III)TPPS4. probably because its cationic charge favors association with the mitochondrial membrane. This is supported by the decrease in the association constant of Fe(III)TMPyP with mitochondria in higher salt medium. (c) 2006 Elsevier Inc. All rights reserved.
dc.description457
dc.description2
dc.description217
dc.description224
dc.languageen
dc.publisherElsevier Science Inc
dc.publisherNew York
dc.publisherEUA
dc.relationArchives Of Biochemistry And Biophysics
dc.relationArch. Biochem. Biophys.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectisolated mitochondria
dc.subjectmitochondrial permeability transition
dc.subjectreactive oxygen species
dc.subjectcalcium
dc.subjectcationic porphyrin
dc.subjectanionic porphyrin
dc.subjectirradiation
dc.subjectphotodynamic therapy
dc.subjectInner Membrane Permeabilization
dc.subjectPhotodynamic Therapy
dc.subjectSubcellular-localization
dc.subjectFibrosarcoma Cells
dc.subjectBiological-systems
dc.subjectOxidative Stress
dc.subjectPorphyrins
dc.subjectApoptosis
dc.subjectPhotosensitization
dc.subjectPhosphorylation
dc.titleIrradiated cationic mesoporphyrin rat liver mitochondria induces larger damage to isolated than the anionic form
dc.typeArtículos de revistas


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